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Structure of the λ Operators


The two regions of λ DNA to which the λ repressor binds (the operators) are shown to consist of multiple, non-identical repressor binding sites. The arrangement of these sites with respect to the adjacent controlled genes has been determined using a restriction endonuclease that cleaves each operator.

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  1. Ptashne, M., in The Bacteriophage Lambda (edit. by Hershey, A.), 221 (Cold Spring Harbor Laboratory, New York, 1971).

    Google Scholar 

  2. Maniatis, T., Ptashne, M., and Maurer, R., Cold Spring Harb. Symp. quant. Biol., 38 (in the press).

  3. Pirrotta, V., Nature new Biol., 244, 13 (1973).

    Article  CAS  Google Scholar 

  4. Maniatis, T., and Ptashne, M., Proc. nat. Acad. Sci. U.S.A., 70, 1531 (1973).

    Article  ADS  CAS  Google Scholar 

  5. Sharpe, P. A., Sugden, B., and Sambrook, J., Biochemistry, N.Y., 12, 3055 (1973).

    Article  Google Scholar 

  6. Allet, B., Biochemistry, N.Y., 12, 3972 (1973).

    Article  CAS  Google Scholar 

  7. Gilbert, W., and Maxam, A., Proc. nat. Acad. Sci. U.S.A. (in the press).

  8. Gilbert, W., and Muller-Hill, B., Proc. natn. Acad. Sci. U.S.A., 58, 2415 (1967).

    Article  ADS  CAS  Google Scholar 

  9. Riggs, A. O., Suzuki, J., and Bourgeois, S., J. molec. Biol., 48, 67 (1970).

    Article  CAS  Google Scholar 

  10. Pirrotta, V., Chadwick, P., and Ptashne, M., Nature, 227, 41 (1970).

    Article  ADS  CAS  Google Scholar 

  11. Chadwick, P., Pirrotta, V., Steinberg, R., Hopkins, N., and Ptashne, M., Cold Spring Harb. Symp. quant. Biol., 35, 283 (1970).

    Article  CAS  Google Scholar 

  12. Danna, K., and Nathans, D., Proc. natn. Acad. Sci. U.S.A., 68, 2913 (1971).

    Article  ADS  CAS  Google Scholar 

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MANIATIS, T., PTASHNE, M. Structure of the λ Operators. Nature 246, 133–136 (1973).

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