Abstract
The two regions of λ DNA to which the λ repressor binds (the operators) are shown to consist of multiple, non-identical repressor binding sites. The arrangement of these sites with respect to the adjacent controlled genes has been determined using a restriction endonuclease that cleaves each operator.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
References
Ptashne, M., in The Bacteriophage Lambda (edit. by Hershey, A.), 221 (Cold Spring Harbor Laboratory, New York, 1971).
Maniatis, T., Ptashne, M., and Maurer, R., Cold Spring Harb. Symp. quant. Biol., 38 (in the press).
Pirrotta, V., Nature new Biol., 244, 13 (1973).
Maniatis, T., and Ptashne, M., Proc. nat. Acad. Sci. U.S.A., 70, 1531 (1973).
Sharpe, P. A., Sugden, B., and Sambrook, J., Biochemistry, N.Y., 12, 3055 (1973).
Allet, B., Biochemistry, N.Y., 12, 3972 (1973).
Gilbert, W., and Maxam, A., Proc. nat. Acad. Sci. U.S.A. (in the press).
Gilbert, W., and Muller-Hill, B., Proc. natn. Acad. Sci. U.S.A., 58, 2415 (1967).
Riggs, A. O., Suzuki, J., and Bourgeois, S., J. molec. Biol., 48, 67 (1970).
Pirrotta, V., Chadwick, P., and Ptashne, M., Nature, 227, 41 (1970).
Chadwick, P., Pirrotta, V., Steinberg, R., Hopkins, N., and Ptashne, M., Cold Spring Harb. Symp. quant. Biol., 35, 283 (1970).
Danna, K., and Nathans, D., Proc. natn. Acad. Sci. U.S.A., 68, 2913 (1971).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
MANIATIS, T., PTASHNE, M. Structure of the λ Operators. Nature 246, 133–136 (1973). https://doi.org/10.1038/246133a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/246133a0
This article is cited by
-
Deep learning for DNase I hypersensitive sites identification
BMC Genomics (2018)
-
Global mapping of protein-DNA interactions in vivo by digital genomic footprinting
Nature Methods (2009)
-
Mutational analysis of the operators of bacteriophage lambda
Molecular and General Genetics MGG (1978)
-
Sequence of the OR operator of phage λ
Nature (1975)
-
Propeties of plasmids produced by recombination between R factors of groups J and FII
Molecular and General Genetics MGG (1975)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.