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An Anti-Inflammatory Peptide from Bee Venom


THE belief that the venom of the common honey bee, Apis mellifira, is beneficial in certain arthritic and rheumatoid conditions goes back to antiquity1. In a study of phospholi-pase-A2,3 we have fractionated nearly 1 kg of freeze-dried bee venom and tested all the fractions for anti-inflammatory activity. Preliminary experiments showed activity to be associated with the basic peptide fraction. The peptides were separated using ‘Sephadex’ G25 and G50 followed by ion-exchange chromatography using either CM ‘Sephadex’ C25 (pH 4.7) or SE ‘Sephadex’ C25 (pH 4.2).

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BILLINGHAM, M., MORLEY, J., HANSON, J. et al. An Anti-Inflammatory Peptide from Bee Venom. Nature 245, 163–164 (1973).

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