Figure 1: Indigo reduction by Clostridium isolated from a woad vat. | Nature

Figure 1: Indigo reduction by Clostridium isolated from a woad vat.

From: Clostridium used in mediaeval dyeing

Figure 1

The plates were initially brown because, even though they contained indigo, the dye was not divided finely enough to colour the agar blue. Clostridium reduced the indigo anaerobically to its colourless form, then, when exposed to air, the indigo was reoxidized and the agar turned blue.

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