Abstract
I HAVE found that several aspects of the morphology and biochemistry of cultured foetal rat brain cells can be altered by N-monobutyryl-cyclic 3′,5′ monophosphate (N-but cyclic AMP). Dispersed cell cultures of foetal rat brain were grown for 15 days in the presence of 10−3 M N-but cyclic AMP and compared with similar untreated cultures. N-but cyclic AMP, prepared from dibutyryl cyclic AMP (Sigma) as previously described1, was used because of its expected greater activity than cyclic AMP in an intact cell preparation2. It is more stable than the dibutyryl form which may release appreciable quantities of butyric acid1.
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SHAPIRO, D. Morphological and Biochemical Alterations in Foetal Rat Brain Cells cultured in the Presence of Monobutyryl Cyclic AMP. Nature 241, 203–204 (1973). https://doi.org/10.1038/241203a0
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DOI: https://doi.org/10.1038/241203a0
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