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  • Original Article
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Lymphoma

Cell origin of pyothorax-associated lymphoma: a lymphoma strongly associated with Epstein–Barr virus infection

Leukemia volume 22, pages 620–627 (2008)Cite this article

Abstract

Pyothorax-associated lymphoma (PAL) is an Epstein–Barr virus (EBV)-associated B cell lymphoma developing in the pleural cavity affected by chronic pyothorax. To clarify the cell origin of PAL, the expression of immunoglobulin heavy (IgH) and light chains in relation to somatic hypermutations (SHMs) of rearranged Ig heavy- and light-chain variable (IgVH, IgVL) genes was examined using cell lines as well as clinical samples. SHMs without ongoing mutations of the IgVH gene were found in all PAL cell lines and clinical samples available for sequencing, indicating PAL to be derived from B cells at the postgerminal center (GC) stage of the differentiation process. They could be subdivided into post-GC cells with potentially productive IgVH genotypes (Group 1) and with sterile IgVH genotypes (Group 2). IgH expression was abrogated in Group 2 as expected and also in two cell lines in Group 1. DNA demethylation experiments with 5-aza-dC induced expression of IgH mRNA and protein in these cell lines. Most PAL cells were derived from crippled post-GC cells, which usually could not survive. Transformation of such B cells through EBV infection might provide a basis for the development of PAL with additional genetic changes.

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Acknowledgements

This study was supported by grants from the Ministry of Education, Science, Culture, and Sports, Japan (14031213, 14770073, 15026209, 15406013, 15590340, 16390105 and 18014015).

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Authors and Affiliations

  1. Department of Pathology, Osaka University Graduate School of Medicine, Osaka, Japan

    T Takakuwa, K Tresnasari, N Rahadiani, H Miwa & K Aozasa

  2. Department of Hematology and Respiratory Medicine, Kochi Medical School, Kochi University, Kochi, Japan

    M Daibata

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  1. T Takakuwa
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  4. H Miwa
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Correspondence to T Takakuwa.

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Takakuwa, T., Tresnasari, K., Rahadiani, N. et al. Cell origin of pyothorax-associated lymphoma: a lymphoma strongly associated with Epstein–Barr virus infection. Leukemia 22, 620–627 (2008). https://doi.org/10.1038/sj.leu.2405059

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