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Modulation of cell apoptosis by AIR

Leukemia volume 21, pages 2557–2559 (2007)Cite this article

A Corrigendum to this article was published on 16 January 2008

As illustrated in the following description, AIR antisense oligodeoxynucleotides (ODN) or transfection with an AIR-carrying pcDNA resulted in, respectively, reducing or enhancing apoptosis, indicating that AIR activity in the cell death program can be worth of investigation and its targeting can modulate the process.

In Figure 1B, the effect of an AIR-specific phosphorothioate antisense ODN on Jurkat cell apoptosis is depicted. In cells incubated with DEM (1 mM), the addition of AIR antisense ODN to the cell culture prevented mitochondrial membrane depolarization, while the control, scrambled ODN did not modify the flow cytometer image. Cytochrome c release in cell cytosol and annexin V binding to the cells were also analysed. Cell incubation with DEM resulted in raising the cytosolic levels of cytochrome c and the percentage of annexin V-binding cells by more than the 100%. In both cases, cell treatment with antisense, but not with control, ODN inhibited the effects of DEM (Figure 1B). The pro-apoptotic effect of AIR antisense ODN was also observed in a primary culture of cells from a patient affected by acute myeloid leukaemia. In these cells, AIR protein was detectable by immunofluorescence; its levels were effectively downmodulated by AIR antisense ODN (Figure 1C, a). AIR downmodulation resulted in increasing caspase 3 activity (Figure 1C, b) and cell apoptosis (Figure 1C, c).

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, University of Salerno, Salerno, Italy

    M C Turco, A Petrella, M Ammirante, A Rosati & M d' Avenia

  2. Department of Biochemistry of Biochemical Medicine, Federico II University, Naples, Italy

    A Lamberti, R Bisogni & M F Romano

  3. Animal Facility, Tumor Institute ‘Fond. Pascale’, Naples, Italy

    C Arra

  4. SAFU Department, Regina Elena Tumor Institute, Rome, Italy

    E Spugnini

  5. Department of Experimental and Clinical Medicine, University of Catanzaro, Catanzaro, Italy

    S Venuta

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  1. M C Turco
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  2. A Lamberti
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Correspondence to M C Turco.

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Turco, M., Lamberti, A., Bisogni, R. et al. Modulation of cell apoptosis by AIR. Leukemia 21, 2557–2559 (2007). https://doi.org/10.1038/sj.leu.2404837

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