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No nucleophosmin mutations in pediatric acute myeloid leukemia with normal karyotype: a study of the Japanese Childhood AML Cooperative Study Group

Leukemia volume 21, page 1307 (2007)Cite this article

Acute myeloid leukemia (AML) with normal karyotype had a heterogenous prognosis. In this subgroup, FLT3-internal tandem duplication (ITD) was strongly associated with a poor prognosis.1, 2, 3 Recently, it was reported that mutations of nucleophosmin (NPM) gene occur in 50–60% of adult AML with normal karyotype and were frequently associated with FLT3-ITD. In the AML patients with normal karyotype and FLT3-ITD, patients with NPM gene mutations showed a better prognosis than those without NPM gene mutations.4, 5, 6 However, the frequency and clinical impact of NPM gene mutations in pediatric AML patients with normal karyotype remained uncertain because there were a few number of reports.7, 8

We searched for NPM gene mutations in 33 (20.9%) of 158 patients with normal karyotype who were treated on Japanese Childhood AML Cooperative protocol, AML 99 (0–15 years old, median 8 years old).9 We amplified exon 12 of NPM gene using the primers; NPM cDNA Fow, 5′-AAAGGTGGTTCTCTTCCCAAA-3′ and NPM cDNA Rev, 5′-GCATTATAAAAAGGACAGCCAGA-3′ and directly sequenced on a DNA sequencer (ABI 310; Applied Biosystems, Foster City, CA, USA) using a BigDye terminator cycle sequencing kit (Applied Biosystems).9 We could not find any NPM gene mutations in this study.

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Acknowledgements

This work was supported in part by a Grant-in-Aid for Cancer Research, a Grant for Clinical Cancer Research and Research on Children and Families from the Ministry of Health, Labor and Welfare of Japan. This work was also supported by Grant-in-Aid for Scientific Research (C) from the Ministry of Education, Culture, Sports, Science and Technology of Japan and Research Grant for Gunma Prefectural Hospitals.

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Authors and Affiliations

  1. Department of Hematology/Oncology, Gunma Children's Medical Center, Hokkitsu, S, hibukawa, Gunma, Japan

    A Shimada, C Kubota & Y Hayashi

  2. Department of Molecular Laboratory Medicine, Kyoto Prefectural University of Medicine Graduate School of Medical Science, Kamigyo-ku, Kyoto, Japan

    T Taki

  3. Department of Pediatrics, National Hospital Organization Osaka National Hospital, Chuo-ku, Osaka, Japan

    A Tawa

  4. Clinical Research Center,

    K Horibe

  5. , National Hospital Organization Nagoya Medical Center, Naka-ku, Nagoya, Japan

    K Horibe

  6. Department of Pediatrics, Ibaraki Children’s Hospital, Mito, Ibaraki, Japan

    M Tsuchida

  7. Division of Hematology/Oncology, Saitama Children’s Medical Center, Saitama, Saitama, Japan

    R Hanada

  8. Department of the First Pediatrics, Toho University School of Medicine, O, ota-ku, Tokyo, Japan

    I Tsukimoto

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  1. A Shimada
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Correspondence to Y Hayashi.

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Shimada, A., Taki, T., Kubota, C. et al. No nucleophosmin mutations in pediatric acute myeloid leukemia with normal karyotype: a study of the Japanese Childhood AML Cooperative Study Group. Leukemia 21, 1307 (2007). https://doi.org/10.1038/sj.leu.2404625

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