Figure 2 | Leukemia

Figure 2

From: High expression of several tyrosine kinases and activation of the PI3K/AKT pathway in mediastinal large B cell lymphoma reveals further similarities to Hodgkin lymphoma

Figure 2

Sensitivity of Karpas1106P and MedB-1 towards the PI3K inhibitor LY294002. Karpas1106P, MedB-1 and for comparison also the LY294002 sensitive T-cell leukemia-derived cell line Jurkat were incubated at 1 × 105 cells/ml with various concentrations of LY294002 for 24 h. Survival was monitored with a MTT assay (based on the activity of the mitochondrial respiratory chain) (a). Western blot analysis with a phospho-AKT-specific antibody (b) showed that treatment of the three cell lines with LY294002 for 24 h before lysis significantly reduced the amount of phosphorylated AKT, indicating that the effect of LY294002 on the three cell lines is largely owing to inhibition of PI3K.

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