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The JAK2V617F mutation is detectable in granulocyte populations at greater than 2 copies per cell among individuals with myeloproliferative disorders

Leukemia volume 21, page 818 (2007)Cite this article

We thank Hammond et al. for having provided technical arguments explaining why they consider the finding of mutant JAK2 copies in normal granulocytes as polymerase chain reaction (PCR) artefacts.1 According to the technical details given, we can understand why the discrimination between the wild-type and mutant JAK2 allele is not absolute as it is only based on the specificity of the 3′-end base of a primer. Our methodology is clearly different.2 Furthermore, the positive results that we obtained were highly reproducible: in patients who showed to be initially positive, the mutation was recurrently found in repeated PCR performed on both genomic DNA and cDNA preparations. Moreover, some of the positive PCR results were confirmed by sequencing. Therefore, we have good reasons to consider that the JAK2V617F mutation found in some of our normal patients is not a PCR artefact.

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References

  1. Hammond E, Shaw K, Herrmann R . Reply in reply to &# × 00AB: The JAK2 V617F mutation is detectable in granulocyte populations at greater than 2 copies per cell among individuals with myeloproliferative disorders. Leukemia (published online).

  2. Sidon P, Heimann P, Lambert F, Dessars B, Robin V, El Housni H . Combined locked nucleic acid and molecular beacon technologies for sensitive detection of the JAK2V617F somatic single-base sequence variant. Clin Chem 2006; 52: 1436–1438.

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Authors and Affiliations

  1. Department of Medical Genetics, Faculty of Medicine, Free University of Brussels, Brussels, Belgium. E-mail: pheimann@ulb.ac.be,

    H E L Housni, P Sidon, B Dessars & P Heimann

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  1. H E L Housni
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  2. P Sidon
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  3. B Dessars
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  4. P Heimann
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Housni, H., Sidon, P., Dessars, B. et al. The JAK2V617F mutation is detectable in granulocyte populations at greater than 2 copies per cell among individuals with myeloproliferative disorders. Leukemia 21, 818 (2007). https://doi.org/10.1038/sj.leu.2404570

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