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Characterization of an artificial antigen-presenting cell to propagate cytolytic CD19-specific T cells

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References

  1. Brentjens RJ, Latouche JB, Santos E, Marti F, Gong MC, Lyddane C et al. Eradication of systemic B-cell tumors by genetically targeted human T lymphocytes co-stimulated by CD80 and interleukin-15. Nat Med 2003; 9: 279–286.

    Article  CAS  Google Scholar 

  2. Imai C, Mihara K, Andreansky M, Nicholson IC, Pui CH, Geiger TL et al. Chimeric receptors with 4-1BB signaling capacity provoke potent cytotoxicity against acute lymphoblastic leukemia. Leukemia 2004; 18: 676–684.

    Article  CAS  Google Scholar 

  3. Cooper LJ, Topp MS, Serrano LM, Gonzalez S, Chang WC, Naranjo A et al. T-cell clones can be rendered specific for CD19: toward the selective augmentation of the graft-versus-B-lineage leukemia effect. Blood 2003; 101: 1637–1644.

    Article  CAS  Google Scholar 

  4. Serrano LM, Pfeiffer T, Olivares S, Numbenjapon T, Bennitt J, Kim D et al. Differentiation of naïve cord blood T cells into CD19-specific cytolytic effectors for post-transplant adoptive immunotherapy. Blood 2006; 107: 2643–2652.

    Article  CAS  Google Scholar 

  5. Riddell SR, Greenberg PD . The use of anti-CD3 and anti-CD28 monoclonal antibodies to clone and expand human antigen-specific T cells. J Immunol Methods 1990; 128: 189–201.

    Article  CAS  Google Scholar 

  6. Maus MV, Thomas AK, Leonard DG, Allman D, Addya K, Schlienger K et al. Ex vivo expansion of polyclonal and antigen-specific cytotoxic T lymphocytes by artificial APCs expressing ligands for the T-cell receptor, CD28 and 4-1BB. Nat Biotechnol 2002; 20: 143–148.

    Article  CAS  Google Scholar 

  7. Cooper LJ, Al-Kadhimi Z, Serrano LM, Pfeiffer T, Olivares S, Castro A et al. Enhanced antilymphoma efficacy of CD19-redirected influenza MP1-specific CTLs by cotransfer of T cells modified to present influenza MP1. Blood 2005; 105: 1622–1631.

    Article  CAS  Google Scholar 

  8. Rossig C, Bar A, Pscherer S, Altvater B, Pule M, Rooney CM et al. Target antigen expression on a professional antigen-presenting cell induces superior proliferative antitumor T-cell responses via chimeric T-cell receptors. J Immunother 2006; 29: 21–31.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We thank Lucy Brown, Claudia Spalla and Chy-Anh Tran for help with flow cytometry and cell sorting, Dr David Smith for help with statistical analyses and Dr Wichai Prayoonwiwat from Thailand for support and advice. This work was supported by, CA30206, CA003572, CA107399, Phramongkutklao Royal Thai Army Hospital and College of Medicine, The Alliance for Cancer Gene Therapy, The Amy Phillips Charitable Foundation, The Leukemia and Lymphoma Society, The Lymphoma Research Foundation, The National Foundation for Cancer Research, The Pediatric Cancer Research Foundation, The National Marrow Donor Program and The Marcus Foundation.

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Numbenjapon, T., Serrano, L., Singh, H. et al. Characterization of an artificial antigen-presenting cell to propagate cytolytic CD19-specific T cells. Leukemia 20, 1889–1892 (2006). https://doi.org/10.1038/sj.leu.2404329

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