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High expression of EDAG and its significance in AML

Leukemia volume 19, pages 1499–1502 (2005)Cite this article

TO THE EDITOR

Embryonic develop associated gene (EDAG), the human HEMGN gene, encodes a novel nuclear protein, which was first identified in 2001. It is specifically expressed in human hematopoietic tissues and cells such as adult bone marrow, CD34+ progenitor cells and leukemia cell line K562. No expression was detected in cultured T cells, monocytes or other nonhematopoietic cell lines.1 Downregulation of EDAG expression also occurs during erythroid differentiation of K562 cells. Overexpression of EDAG in NIH3T3 cells causes cell transforming and tumor forming in nude mice.2 Furthermore, EDAG gene maps to chromosome 9q22, which contains a cluster of leukemic breakpoints and genes important for leukemogenesis.1 However, its clinical significance and function in leukemogenesis is still unclear. In the present study, we detected EDAG mRNA expression in a panel of leukemia cell lines and the bone marrow mononuclear cells (BMMC) of 75 acute myeloid leukemia (AML) patients to explore the clinical significance of EDAG. Then we investigated the effect of EDAG gene knockdown by RNA interference (RNAi) on leukemia cell line K562 to study the molecular mechanism.

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Authors and Affiliations

  1. State Key Laboratory for Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, PR China

    L-L An, G Li, K-F Wu, X-T Ma, G-G Zheng, L-G Qiu & Y-H Song

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  1. L-L An
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  2. G Li
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Correspondence to K-F Wu.

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An, LL., Li, G., Wu, KF. et al. High expression of EDAG and its significance in AML. Leukemia 19, 1499–1502 (2005). https://doi.org/10.1038/sj.leu.2403808

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