Ex vivo expansion of CD34+ cells in a contact coculture system with primary AGM cells as the stromal layer. Enriched CD34+ cells were cultured for 7 and 14 days in the presence of cytokines T6F, with or without pre-established AGM cells. AGM cells promoted the expansion of various stem and progenitor cell populations. n=9. *P⩽0.05, **P⩽0.01.