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Activation-induced cytidine deaminase expression in follicular lymphoma: association between AID expression and ongoing mutation in FL

Leukemia volume 18, pages 826–831 (2004)Cite this article

Abstract

Activation-induced cytidine deaminase (AID) is required for somatic hypermutation (SHM) and class switch recombination (CSR) of the immunoglobulin (Ig) gene. AID has been reported to be specifically expressed in the germinal center (GC). Follicular lymphoma (FL) cells are known to be exposed to GC reaction, as characterized by a high degree of SHM with some heterogeneity in terms of intraclonal microheterogeneity and antigen selection. The heterogeneity of SHM pattern in FL intrigued us to investigate the AID expression. AID expression was investigated in 19 FL materials consisting of 15 cases of FL fresh cells and four cell lines. In all, 10 fresh cells and three cell lines expressed AID, but the others did not. SHM was investigated in 12 fresh cells and four cell lines. The ongoing mutation was significantly different between AID-positive and AID-negative FL fresh cells (unpaired Student's t-test, P=0.047). Ongoing mutation was not seen in any of the cell lines. AID expression was associated with the ongoing mutation in FL fresh cells (two-tailed Pearson's coefficient correlation, r=0.899, P=0.01). The switch off of AID expression may start in the B-lineage differentiation stage counterpart of FL after optimizing SHM, indicated by the cessation of the ongoing mutation in AID-negative FL fresh cells.

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Article 12 December 2019

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Acknowledgements

MSH is a Graduate Student of Foreign Scholarship of Japan (MONBUSHO) under the superiority of Professor Masafumi Matsuo.

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Authors and Affiliations

  1. International Center for Medical Research (ICMR), Graduate School of Medicine, Kobe University, Kobe, Japan

    M S Hardianti, E Tatsumi, M Syampurnawati & K Furuta

  2. Blood Transfusion Division, Graduate School of Medicine, Kobe University, Japan

    K Saigo

  3. Department of Laboratory Medicine, Graduate School of Medicine, Kobe University, Japan

    Y Nakamachi & S Kumagai

  4. Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University, Kyoto, Japan

    H Ohno

  5. Department of Hematology, Nara Social Health Insurance Hospital, Nara, Japan

    H Ohno

  6. Department of Hematology, Hyogo Prefectural Amagasaki Hospital, Amagasaki, Japan

    S Tanabe & M Uchida

  7. Department of Internal Medicine, Torigata Hospital, Osaka, Japan

    S Tanabe

  8. Department of General Internal Medicine, Kyoto City Hospital, Kyoto, Japan

    N Yasuda

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  1. M S Hardianti
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Correspondence to E Tatsumi.

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Hardianti, M., Tatsumi, E., Syampurnawati, M. et al. Activation-induced cytidine deaminase expression in follicular lymphoma: association between AID expression and ongoing mutation in FL. Leukemia 18, 826–831 (2004). https://doi.org/10.1038/sj.leu.2403323

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  • DOI: https://doi.org/10.1038/sj.leu.2403323

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