Table 4 Comparison between different fixation times

From: Simultaneous detection of the immunophenotypic markers and genetic aberrations on routinely processed paraffin sections of lymphoma samples by means of the FICTION technique

PC minutes Case of normal tonsil and FCL using ALK and BCL2/IGH probes 12 h 24 h 48 h 1 week 1 h B5
2 min FISH ++ ++ ± +
  Immunofluorescence + ++ ± ± ±
4 min FISH ++ ++ ± +
  Immunofluorescence + + ± ±
8 min FISH ++ + ± +
  Immunofluorescence + + ± + ±
  1. ++=Good.
  2. +=Sufficient.
  3. ±=Not good.
  4. −=Negative.