Table 3 Comparison between different buffers and pressure-cooking times

From: Simultaneous detection of the immunophenotypic markers and genetic aberrations on routinely processed paraffin sections of lymphoma samples by means of the FICTION technique

PC minutes Case of ALCL (ALK+) EDTA, pH 8 Tris-EDTA, pH 9 Citrate, pH 6.5
2 min Nuclear morphology ++ ++ +
  FISH intensity ++ ++ ++
  Immunofluorescence intensity ++ ++ ±
  Tissue architecture ++ ++ +
  Background No No Yes
4 min Nuclear morphology + + +
  FISH intensity ++ ++ ++
  Immunofluorescence intensity + + ±
  Tissue architecture ++ ++ +
  Background No No Yes
8 min Nuclear morphology ± ± ±
  FISH intensity ± ++ ++
  Immunofluorescence intensity + + +
  Tissue architecture + ++ +
  Background Yes No Yes
  1. ++=Good.
  2. +=Sufficient.
  3. ±=Not good.
  4. For this experiment, we have used a case of ALCL double stained with CD20 and CD30 monoclonal antibodies using immunofluorescence in combination with the break-apart ALK FISH probe (as described in Table 2).