Abstract
To accurately estimate the incidence of HOX11L2 expression, and determine the associated cytogenetic features, in T-cell acute lymphoblastic leukemia (T-ALL), the Groupe Français de Cytogénétique Hématologique (GFCH) carried out a retrospective study of both childhood and adult patients. In total, 364 patients were included (211 children ⩽15 years and 153 adults), and 67 (18.5%) [47 children (22.4%) and 20 adults (13.1%)] were shown to either harbor the t(5;14)q35;q32) translocation or express the HOX11L2 gene or both. Most of the common hematological parameters did not show significant differences within positive and negative populations, whereas the incidence of CD1a+/CD10+ and cytoplasmic CD3+ patients was significantly higher in positive than in negative children. Out of the 63 positive patients investigated by conventional cytogenetics, 32 exhibited normal karyotype, whereas the others 31 showed clonal chromosome abnormalities, which did not include classical T-ALL specific translocations. Involvement of the RANBP17/HOX11L2 locus was ascertained by fluorescence in situ hybridization in six variant or alternative (three-way translocation or cytogenetic partner other than 14q32) translocations out of the 223 patients. Our results also show that HOX11L2 expression essentially occurs as a result of a 5q35 rearrangement, but is not associated with another identified T-ALL specific recurrent genetic abnormality, such as SIL-TAL fusion or HOX11 expression.
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Acknowledgements
The Belgian contribution to this work presents research results of the Belgian Programme of Interuniversity Poles of Attraction initiated by the Belgian State, Prime Minister's Office, Science Policy Programming. We own scientific responsibility of this work.
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Appendix
The Appendix lists the name of the city, the name of the institute, followed by the names of other participants and the number of cases in parentheses.
Toulouse: Centre Hospitalier Universitaire Hôpital Purpan: EE Kuhlein, E Duchayne, A Robert, F Huguet (53).
Paris: Hôpital Trousseau et CHU Saint Antoine: M Adam, L Douay, J Landman-Parker, G Leverger (45).
Paris: Hôpital Saint Louis: X-Y Su, M-T Daniel (40).
Louvain: Katholieke Universiteit Leuven: A Uyttebroek, C Doyen, A Delannoy, B Deprijck; Université Catholique de Louvain: C Verellen-Dumoulin, JM Libouton, JP Scheiff, V Deneys, C Vermylen, A Bruwier, D Latinne, J-L Vaerman, A Ferrant, A Bruniez (32).
Lyon: Hôpital Edouard Herriot and Hôpital Debrousse: I Tigaud, E Callet-Bauchu, D Treille-Ritouet, X Thomas (30).
Dijon: Centre Hopitalier Universitaire: B Favre, M Maynadié, D Caillo (25).
Lille: Centre Hospitalier Universitaire: P Lepelley, C Roumieux, N Grardel (18).
Nantes: Centre Hospitalier Universitaire Hôtel-Dieu: H Hervé-Loiseau (18).
Strasbourg: Centre Hospitalier Régional Universitaire: M Lessard, V Leymarie, C Gervais (18).
Marseille: Institut Paoli-Calmettes: D Sainty, C Arnoulet, R Bouabdallah, D Coso, A Charbonnier, D Blaise, A-M Stoppa, N Vey (14).
Reims: Centre Hospitalier Universitaire: I Luquet, S Daliphard (14).
Paris: Centre Hospitalier Universitaire Necker-Enfants Malades: S Romana (12).
Bordeaux: Centre Hospitalier Universitaire Hôpital Haut Lévêque: J-P Vial, F Lacombe, J-M Boiron (11).
Paris: Hôpital Pitié-Salpétrière: H Merle-Béral, CERBA: H Mossafa (11).
Gand: Centre for Medical Genetics, Belgique: J Philippé, B Verhasselt, Y Benoit (8).
Rouen: Centre Anticancéreux Henri Becquerel: A Stamboulas, MP Callat (7).
Marseille: Hôpital Timone-Enfants: H Zattara (3).
Liège: Université de Liège, ULCB, Belgique (3).
Nancy: Centre Hospitalier Universitaire de Nancy Brabois: P Jonveaux, J Buisine, L Mansury (2).
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Berger, R., Dastugue, N., Busson, M. et al. t(5;14)/HOX11L2-positive T-cell acute lymphoblastic leukemia. A collaborative study of the Groupe Français de Cytogénétique Hématologique (GFCH). Leukemia 17, 1851–1857 (2003). https://doi.org/10.1038/sj.leu.2403061
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DOI: https://doi.org/10.1038/sj.leu.2403061
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