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Functional characterization of human CD34+ cells that express low or high levels of the membrane antigen CD111 (nectin 1)

Leukemia volume 17, pages 1137–1145 (2003)Cite this article

Abstract

Nectins are recently described adhesion molecules that are widely expressed on many tissues, including the hematopoietic tissue. Nectin 1 (CD111) is expressed on a higher proportion of mobilized peripheral blood (mPB) than cord blood (CB) CD34+ cells, and of CD34+/CD38+ cells when compared with CD34+/CD38− cells. We studied functional properties of human CB and mPB CD34+ cells that express low or high levels of CD111. CD34+/CD111dim cells contain a higher proportion of cells in G0/G1 phase than CD34+/CD111bright cells. CD34+/CD111bright cells contain more erythroid progenitors: CFU-E, than their counterparts, which on the opposite contain more HPP-CFC. Limiting dilution analyses demonstrate a higher frequency of immature progenitors: cobblestone-area colony-forming cells, in CD34+/CD111dim than in CD34+/CD111bright cells. In vitro differentiation assays demonstrate a higher frequency of B-, T- and dendritic-cell precursors, but less NK-cell precursors in CD34+/CD111dim cells. Evaluation of engraftment in NOD-SCID mice shows that SCID repopulating cells are more frequent among mPB CD34+/CD111dim cells. Liquid culture of CD34+/CD111dim cells with erythropoietin shows that CD111 expression increases simultaneously with CD36, following CD71 and before glycophorin A expression. In conclusion, immature human hematopoietic progenitors express low levels of CD111 on their surface. During erythroid differentiation CD34+ cells acquire higher levels of the CD111 antigen.

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Acknowledgements

This work was supported in part by Institut Paoli-Calmettes, and by a grant from the Comité Leucémie de la Fondation de France (# 990038) to CC. GB is the recipient of a grant from a joint research training program established by the French and Algerian governments. We thank all personnel at the Tumor Cell Collection (Biothéque) and at the Centre de Thérapie Cellulaire et Génique for access to apheresis and cord blood samples. We also thank Rémy Galindo at the Flow Cytometry Facility, and Patrick Gibier at the animal facility for their help during the conduct of these studies.

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  1. Centre de Thérapie Cellulaire et Génique, Institut Paoli-Calmettes, Centre Régional de Lutte Contre le Cancer Provence-Alpes-Côte d'Azur, Marseille, France

    G Belaaloui, A-M Imbert, F Bardin, C Tonnelle & C Chabannon

  2. Equipe d'Hématopoïese Moléculaire et Fonctionnelle, Inserm U119 : Cancérologie Expérimentale, Marseille, France

    P Dubreuil & M Lopez

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  1. G Belaaloui
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Belaaloui, G., Imbert, AM., Bardin, F. et al. Functional characterization of human CD34+ cells that express low or high levels of the membrane antigen CD111 (nectin 1). Leukemia 17, 1137–1145 (2003). https://doi.org/10.1038/sj.leu.2402916

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