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Appendix: Method In Focus

Multiparameter flow cytometric quantification of apoptosis-related protein expression

Leukemia volume 17, pages 787–788 (2003)Cite this article

The method is developed for accurate analysis of intracellular proteins in combination with extracellular antigens. The method enables detection and quantification of apoptosis-related proteins in a bulk of cells, but more importantly in very small cell populations as has been demonstrated in this paper for Bcl-2, Bcl-xL, Mcl-1 and Bax expression in de novo AML cells and in minimal residual disease (MRD) cells in BM samples of AML patients at several intervals after start of therapy.

Mononuclear cells from BM samples of de novo patients were isolated by ficoll gradient centrifugation, whereafter residual erythrocytes were lysed with ammonium chloride for 10 min at 4°C and washed.

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References

  1. Campana D, Pui CH . Detection of minimal residual disease in acute leukemia: methodologic advances and clinical significance. Blood 1995; 85: 1416–1434.

    CAS  Google Scholar 

  2. San Miguel JF, Martinez A, Macedo A, Vidriales MB, Lopez-Berges C, Gonzalez M et al. Immunophenotyping investigation of minimal residual disease is a useful approach for predicting relapse in acute myeloid leukemia patients. Blood 1997; 90:2465–2470.

    CAS  PubMed  Google Scholar 

  3. Terstappen LW, Safford M, Konemann S, Loken MR, Zurlutter K, Buchner T et al. Flow cytometric characterization of acute myeloid leukemia. Part II. henotypic heterogeneity at diagnosis. Leukemia 1992; 6: 70–80.

    CAS  PubMed  Google Scholar 

  4. Venditti A, Buccisano F, Del Poeta G, Maurillo L, Tamburini A, Cox C, Battaglia A et al. Level of minimal residual disease after consolidation therapy predicts outcome in acute myeloid leukemia. Blood 2000; 96: 3948–3952.

    CAS  PubMed  Google Scholar 

  5. San Miguel JF, Vidriales MB, Lopez-Berges C, Diaz-Mediavilla J, Gutierrez N, Canizo C et al. Early immunophenotypical evaluation of minimal residual disease in acute myeloid leukemia identifies different patient risk groups and may contribute to postinduction treatment stratification. Blood 2001; 98: 1746–1751.

    Article  CAS  Google Scholar 

  6. San Miguel JF, Ciudad J, Vidriales MB, Orfao A, Lucio P, Porwit-MacDonald A et al. Immunophenotypical detection of minimal residual disease in acute leukemia. Crit Rev Oncol Hematol 1999; 32: 175–185.

    Article  CAS  Google Scholar 

  7. Macedo A, Orfao A, Ciudad J, Gonzalez M, Vidriales B, Lopez-Berges MC et al. Phenotypic analysis of CD34 subpopulations in normal human bone marrow and its application for the detection of minimal residual disease. Leukemia 1995; 9: 1896–1901.

    CAS  PubMed  Google Scholar 

  8. Yin JA, Tobal K . Detection of minimal residual disease in acute myeloid leukemia: methodologies,clinical and biological significance. Br J Haematol 1999; 106: 578–590.

    Article  CAS  Google Scholar 

  9. Scolnik MP, Morilla R, de Bracco MM, Catovsky D, Matutes E . CD34 and CD117 are overexpressed in AML and may be valuable to detect minimal residual disease. Leukemia Res 2002; 26: 615–619.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Haemotology, VU University Medical Centre, Amsterdam, The Netherlands

    A van Stijn, A Kok, M A van der Pol, N Feller, G M J M Roemen, A H Westra, G J Ossenkoppele & G J Schuurhuis

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  1. A van Stijn
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  2. A Kok
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  3. M A van der Pol
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van Stijn, A., Kok, A., van der Pol, M. et al. Multiparameter flow cytometric quantification of apoptosis-related protein expression. Leukemia 17, 787–788 (2003). https://doi.org/10.1038/sj.leu.2402886

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