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Mobilization of peripheral blood progenitor cells with a combination of cyclophosphamide, r-metHuSCF and filgrastim in patients with breast cancer previously treated with chemotherapy

Leukemia volume 17, pages 437–441 (2003)Cite this article

Abstract

The objective of our study was to determine the effect of adding r-metHuSCF to Filgrastim and cyclophosphamide for mobilization of peripheral blood progenitor cells (PBPC), on collection of CD34+ cells and engraftment after autologous stem cell transplant. Twenty-three patients with previously treated stage II–IV breast cancer received cyclophosphamide (3 g/m2), Filgrastim 5 μg/kg daily and r-metHuSCF 20 μg/kg daily. Two PBPC collections were performed on consecutive days starting the day the WBC count was above 7.5 × 103/μl. Collection was performed between days +9 and +12 and the median number of CD34+ cells collected was 9.9 × 106/kg (1.1–53.1) and 6.6 × 106/kg (1.4–33.8) for the first and second apheresis, respectively. Despite being previously treated patients, the target CD34+ cell dose required for SCT was obtained in all patients. SCT was associated with rapid neutrophil and platelet engraftment and a highly significant correlation was observed between the number of CD34+ cells infused and engraftment. Treatment with SCF plus filgrastim was well tolerated, with mild to moderate local skin rash being the most frequently reported adverse event. In conclusion, addition of r-metHuSCF induces mobilization of a large number of CD34+ cells which results in shortening of time to engraftment and hospitalization.

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Acknowledgements

This work was supported in part by AMGEN SA, Spain.

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Authors and Affiliations

  1. Servicio de Hematología y Oncología, Hospital Clínico, Universidad de Valencia, Spain

    F Prósper, C Arbona, A Lluch & J García-Conde

  2. Departamento de Medicina and Centro de Investigaciones del Cáncer, Servicio General de Citometría, Universidad de Salamanca, Spain

    P Menéndez & A Orfao

  3. Servicio de Oncología, Hospital Santa Creu i Sant Pau, Barcelona, Spain

    C Solá & J J López

  4. Servicio de Oncología, Hospital 12 de Octubre, Madrid, Spain

    J Hornedo & H Cortés-Funes

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  1. F Prósper
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Prósper, F., Solá, C., Hornedo, J. et al. Mobilization of peripheral blood progenitor cells with a combination of cyclophosphamide, r-metHuSCF and filgrastim in patients with breast cancer previously treated with chemotherapy. Leukemia 17, 437–441 (2003). https://doi.org/10.1038/sj.leu.2402750

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