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  • Biotechnical Methods Section BTS
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Bio-Technical Methods Section (BTS)

Is t(14;18)(q32;q21) a constant finding in follicular lymphoma? An interphase FISH study on 63 patients

Leukemia volume 17, pages 255–259 (2003)Cite this article

Abstract

The translocation (14;18)(q32;q21) is the hallmark of follicular lymphoma (FL). However, conventional cytogenetics and PCR techniques fail to detect it in at least 10% of cases. In order to evaluate the true incidence of this translocation in FL, we analyzed 63 patients with FL, and 17 patients with diffuse large cell lymphoma (DLCL) corresponding to suspected FL transformations using interphase fluorescence in situ hybridization (FISH). Colocalized signals related to the translocation were observed in 19–92% of cells (median = 51%), corresponding to positivity over the threshold in all (63/63) cases. Similarly, 16/17 possibly secondary DLCL displayed the translocation. Although some cytogenetic changes might be missed by this FISH assay (such as rare insertion, or translocations with other chromosomal partners), our results stress t(14;18)(q32;q21) as an almost constant finding in FL. Our sensitive interphase FISH assay should be of great value to define FL more accurately, namely in patients included into therapeutic trials. Furthermore, this approach could be of interest in (re)defining some types of FL, especially the grade 3 FL which frequently lack t(14;18).

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References

  1. The Non-Hodgkin's Lymphoma Classification Project A clinical trial of the International Lymphoma Study Group classification of non-Hodgkin's lymphoma. Blood, (1997). 89, 3909–3918.

  2. Harris, NL, Jaffe, ES, Diebold, J, Flandrin, G, Muller-Hermelink, HK, Vardiman, J, Lister, TA & Bloomfield, CD The World Health Organization Classification of Neoplasms of the Hematopoietic and Lymphoid Tissues: Report of the Clinical Advisory Commitee Meeting – Airlie House, Virginia, November, 1997. The Hematology Journal, (2000). 1, 53–56.

    Article  CAS  PubMed  Google Scholar 

  3. Macintyre, E, Willerford, D & Morris, SW Non-Hodgkin's Lymphoma: molecular features of B Cell Lymphoma. Education Program Book of the American Society of Hematology, American Society of Hematology: San Francisco (2000). pp180–204.

    Google Scholar 

  4. Skinnider, BF, Horsman, DE, Dupuis, B & Gascoyne, RD Bcl-6 and Bcl-2 protein expression in diffuse large B-cell lymphoma and follicular lymphoma: correlation with 3q27 and 18q21 chromosomal abnormalities. Hum Pathol, (1999). 30, 803–808.

    Article  CAS  PubMed  Google Scholar 

  5. Lopez-Guillermo, A, Cabanillas, F, McDonnell, TI, McLaughlin, P, Smith, T, Pugh, W, Hagemeister, F, Rodriguez, MA, Romaguera, JE, Younes, A, Sarris, AH, Preti, HA & Lee, MS Correlation of bcl-2 rearrangement with clinical characteristics and outcome in indolent follicular lymphoma. Blood, (1999). 93, 3081–3087.

    CAS  PubMed  Google Scholar 

  6. Vaandrager, JW, Schuuring, E, Raap, T, Philippo, K, Kleiverda, K & Kluin, P Interphase FISH detection of BCL2 rearrangement in follicular lymphoma using breakpoint-flanking probes. Genes Chromosomes Cancer, (2000). 27, 85–94.

    Article  CAS  PubMed  Google Scholar 

  7. Poetsch, M, Weber-Matthiesen, K, Plendl, HJ, Grote, W & Schlegelberger, B Detection of the t(14;18) chromosomal translocation by interphase cytogenetics with yeast-artificial-chromosome probes in follicular lymphoma and nonneoplastic lymphoproliferation. J Clin Oncol, (1996). 14, 963–969.

    Article  CAS  PubMed  Google Scholar 

  8. Akiko, T, Ikuo, M, Shinsuke, I, Shohei, Y, Shigeo, H, Kazuhiro, N, Hiroshi, F, Shigeo, N, Masao, S, Ryuzo, U & Masafumi, T Interphase detection of immunoglobulin heavy chain gene translocations with specific oncogene loci in 173 patients with B-cell lymphoma. Cancer Genet Cytogenet, (2001). 129, 1–9.

    Article  Google Scholar 

  9. Mitelman, F, Johansson, B & Mertens, S Mitelman database of catalogue of chromosome aberrations in cancer. (2000). http://cgap.nci.nih.gov/Chromosomes/Mitelman

  10. Knutsen, T Cytogenetic mechanisms in the pathogenesis and progression of follicular lymphoma. Cancer Surv, (1997). 30, 163–192.

    CAS  PubMed  Google Scholar 

  11. Avet-Loiseau, H, Li, JY, Facon, T, Brigaudeau, C, Morineau, N, Maloisel, F, Rapp, MJ, Talmant, P, Trimoreau, F, Jaccard, A, Harousseau, JL & Bataille, R High incidence of translocations t(11;14)(q13;q32) and t(4;14)(p16;q32) in patients with plasma cells malignancies. Cancer Res, (1998). 58, 5640–5645.

    CAS  PubMed  Google Scholar 

  12. Rimokh, R, Gadoux, M, Bertheas, MF, Berger, F, Garoscio, M, Deleage, G, Germain, D & Magaud, JP FVT-1, a novel human transcription unit affected by variant translocation t(2;18)(p11;q21) of follicular lymphoma. Blood, (1993). 81, 136–142.

    CAS  PubMed  Google Scholar 

  13. Bastard, C, Tilly, H, Lenormand, B, Bigorgne, C, Boulet, D, Kunlin, A, Monconduit, M & Piguet, H Translocations involving band 3q27 and Ig gene regions in non-Hodgkin's lymphoma. Blood, (1992). 79, 2527–2531.

    CAS  PubMed  Google Scholar 

  14. Gribben, JG, Saporito, L, Barber, M, Blake, KW, Edwards, RM, Griffin, JD, Freedman, AS & Nadler, LM Bone marrows of Non-Hodgkin's lymphoma patients with a bcl-2 translocation can be purged of polymerase chain reaction-detectable lymphoma cells using monoclonal antibodies and immunomagnetic bead depletion. Blood, (1992). 80, 1083–1089.

    CAS  PubMed  Google Scholar 

  15. Vaandrager, JW, Schuuring, E, Philippo, K & Kluin, PM V(D)J recombinase-mediated transposition of the BCL2 gene to the IgH locus in follicular lymphoma. Blood, (2000). 96, 1647–1652.

    Google Scholar 

  16. Küppers, R, Klein, U, Hansmann, ML & Rajewsky, K Cellular origin of human B-cell lymphomas. N Engl J Med, (1999). 11, 1520–1529.

    Article  Google Scholar 

  17. Buchonnet, G, Lenain, P, Ruminy, P, Lepretre, S, Stamatoullas, A, Parmentier, F, Jardin, F, Duval, C, Tilly, H & Bastard, C Characterization of BCL2-JH rearrangements in follicular lymphoma: PCR detection of 3’BCL2 breakpoints and evidence of a new cluster. Leukemia, (2000). 14, 1563–1569.

    Article  CAS  PubMed  Google Scholar 

  18. Yabumoto, K, Akasaka, T, Muramatsu, M, Kadowaki, M, Hayashi, T, Ohno, H, Fukuhara, S & Okuma, M Rearrangement of the 5’ cluster region of the BCL2 gene in lymphoid neoplasm: a summary of nine cases. Leukemia, (1996). 10, 970–977.

    CAS  PubMed  Google Scholar 

  19. Seite, P, Hillion, J, d'Agay, MF, Berger, R & Larsen, CJ BCL2 complex rearrangement in follicular lymphoma: translocation mbr/JH and deletion in the vcr region of the same BCL2 allele. Oncogene, (1993). 8, 3073–3080.

    CAS  PubMed  Google Scholar 

  20. Yunis, JJ, Frizzera, G, Oken, MM, McKenna, J, Theologides, A & Arnesen, M Multiple recurrent genomic defects in follicular lymphoma. A possible model for cancer. N Engl J Med, (1987). 316, 79–84.

    Article  CAS  PubMed  Google Scholar 

  21. Offit, K, Jhanwar, SC, Ladanyi, M, Filippa, DA & Chaganti, RS Cytogenetic analysis of 434 consecutively ascertained specimens of non-Hodgkin's lymphoma: correlations between recurrent aberrations, histology, and exposure to cytotoxic treatment. Genes Chromosomes Cancer, (1991). 3, 189–201.

    Article  CAS  PubMed  Google Scholar 

  22. Ott, G, Katzenberger, T, Lohr, A, Kindelberger, S, Rudiger, T, Wilhelm, M, Kalla, J, Rosenwald, A, Muller, JG, Ott, MM & Muller-Hermelink, HK Cytomorphologic, immunohistochemical, and cytogenetic profiles of follicular lymphoma: 2 types of follicular lymphoma grade 3. Blood, (2002). 99, 3806–3812.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Hematology Laboratory, University Hospital, Nantes, France

    A Godon, P Talmant & H Avet-Loiseau

  2. Pathology Department, University Hospital, Nantes, France

    A Moreau

  3. Hematology Laboratory, University Hospital, Angers, France

    A Godon, L Baranger-Papot, F Geneviève & M Zandecki

  4. Clinical Hematology Department, University Hospital, Nantes, France

    N Milpied

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  1. A Godon
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  2. A Moreau
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Godon, A., Moreau, A., Talmant, P. et al. Is t(14;18)(q32;q21) a constant finding in follicular lymphoma? An interphase FISH study on 63 patients. Leukemia 17, 255–259 (2003). https://doi.org/10.1038/sj.leu.2402739

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