Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Original Manuscript
  • Published:

Establishment of the B cell precursor acute lymphoblastic leukemia cell line MUTZ-5 carrying a (12;13) translocation

Leukemia volume 15, pages 1471–1474 (2001)Cite this article

Abstract

Continuous leukemia–lymphoma cell lines are important research tools, in particular as starting material for the cloning of recurrent translocations. In 1998, we established the continuous leukemia cell line MUTZ-5 and its two simultaneous sister cell lines MUTZ-6 and MUTZ-7. The primary specimen was obtained from the peripheral blood of a 26-year-old man with B cell precursor acute lymphoblastic leukemia at relapse carrying a t(12;13). The immunoprofile of MUTZ-5 corresponds to that of a precursor B cell. The immunoglobulin heavy chain gene was found to be rearranged. Despite receptor expression, none of the cytokines examined enhanced proliferation; several cytokines had significant inhibitory effects. Giemsa-banding cytogenetics showed the following karyotype which was identical in all three sister cell lines: 45<2n>X, −Y, t(12;13)(p12;q13–14). The karyotype and DNA fingerprinting confirmed the malignant nature and the authenticity of the cell line, excluding cross-contamination with other cells. MUTZ-5 represents a new unique leukemia B cell line; its scientific significance lies in the t(12;13).

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2

Similar content being viewed by others

References

  1. Drexler HG (ed . ). The Leukemia–Lymphoma Cell Line Facts Book Academic Press: San Diego 2000

    Google Scholar 

  2. Drexler HG, MacLeod RAF, Borkhardt A, Janssen JWG . Recurrent chromosomal translocations and fusion genes in leukemia–lymphoma cell lines Leukemia 1995 9: 480–500

    CAS  PubMed  Google Scholar 

  3. Van Dongen JJ, MacIntyre EA, Gabert JA, Delabesse E, Rossi V, Saglio G, Gottardi E, Rambaldi A, Dotti G, Griesinger F, Parreira A, Gameiro P, Diaz MG, Malec M, Langerak AW, San Miguel JF, Biondi A . Standardized RT-PCR analysis of fusion gene transcripts from chromosome aberrations in acute leukemia for detection of minimal residual disease. Report of the BIOMED-1 Concerted Action: investigation of minimal residual disease in acute leukemia Leukemia 1999 13: 1901–1928

    Article  CAS  Google Scholar 

  4. Drexler HG, Matsuo Y . Guidelines for the characterization and publication of human malignant hematopoietic cell lines Leukemia 1999 13: 835–842

    Article  CAS  Google Scholar 

  5. Drexler HG, Dirks WG, MacLeod RAF . False human hematopoietic cell lines: cross-contaminations and misinterpretations Leukemia 1999 13: 1601–1607

    Article  CAS  Google Scholar 

  6. Drexler HG, Dirks WG, MacLeod RAF, Quentmeier H, Steube KG, Uphoff CC (eds) . DSMZ Catalogue of Human and Animal Cell Lines, 8th edn Braunschweig: Germany 2001

    Google Scholar 

  7. Drexler HG, Zaborski M, Quentmeier H . Cytokine response profiles of human myeloid factor-dependent leukemia cell lines Leukemia 1997 11: 701–708

    Article  CAS  Google Scholar 

  8. Van Dongen JJM, Wolvers-Tettero ILM . Analysis of immunoglobulin and T cell receptor genes. Part I: basic and technical aspects Clin Chim Acta 1991 198: 1–92

    Article  CAS  Google Scholar 

  9. Hu ZB, Ma W, Zaborski M, MacLeod RAF, Quentmeier H, Drexler HG . Establishment and characterization of two novel cytokine-responsive acute myeloid and monocytic leukemia cell lines, MUTZ-2 and MUTZ-3 Leukemia 1996 10: 1025–1040

    CAS  PubMed  Google Scholar 

  10. Matsuo Y, MacLeod RAF, Uphoff CC, Drexler HG, Nishizaki C, Katayama Y, Kimura G, Fujii N, Omoto E, Harada M, Orita K . Two acute monocytic leukemia (AML-M5a) cell lines (MOLM-13 and MOLM-14) with interclonal phenotypic heterogeneity showing MLL-AF9 fusion resulting from an occult chromosome insertion, ins(11;9)(q23;p22p23) Leukemia 1997 11: 1469–1477

    Article  CAS  Google Scholar 

  11. Drexler HG, Uphoff CC . Contamination of cell culture, mycoplasma. In: Spier E et al (eds) The Encyclopedia of Cell Technology Wiley: New York 2000 pp 609–627

    Google Scholar 

  12. Bene MC, Castoldi G, Knapp W, Ludwig WD, Matutes E, Orfao A, Van't Veer MB, European Group for the Immunological Characterization of Leukemia . Proposals for the immunological classification of acute leukemias Leukemia 1995 9: 1783–1786

    CAS  PubMed  Google Scholar 

  13. Coignet LJ, Lima CS, Min T, Streubel B, Swansbury J, Telford N, Swanton S, Bowen A, Nagai M, Catovsky D, Fonatsch C, Dyer MJ . Myeloid- and lymphoid-specific breakpoint cluster regions in chromosome band 13q14 in acute leukemia Genes Chromosomes Cancer 1999 25: 222–229

    Article  CAS  Google Scholar 

  14. Bohlander SK . Fusion genes in leukemia: an emerging network Cytogenet Cell Genet 2000 91: 52–56

    Article  CAS  Google Scholar 

  15. Uphoff CC, MacLeod RAF, Denkmann SA, Golub TR, Borkhardt A, Janssen JWG, Drexler HG . Occurrence of TEL-AML1 fusion resulting from (12;21) translocation in human early B-lineage leukemia cell lines Leukemia 1997 11: 441–447

    Article  CAS  Google Scholar 

  16. Ferrando AA, Look AT . Clinical implications of recurring chromosomal and associated molecular abnormalities in acute lymphoblastic leukemia Semin Hematol 2000 37: 381–395

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We thank Dorothé Erz, Dr W Dirks and Dr C Uphoff for performing immunoglobulin rearrangement, DNA fingerprinting and viral/mycoplasmal analysis.

Author information

Authors and Affiliations

  1. DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany

    C Meyer, RAF MacLeod, H Quentmeier & HG Drexler

  2. Institute for Human Genetics, University of Heidelberg, Germany

    JWG Janssen

  3. Cardinal Bernardin Cancer Center, Loyola University Medical School, Maywood, IL, USA

    LJ Coignet

  4. University of Leicester, Leicester, UK

    MJS Dyer

Authors
  1. C Meyer
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. RAF MacLeod
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H Quentmeier
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. JWG Janssen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. LJ Coignet
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. MJS Dyer
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. HG Drexler
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Meyer, C., MacLeod, R., Quentmeier, H. et al. Establishment of the B cell precursor acute lymphoblastic leukemia cell line MUTZ-5 carrying a (12;13) translocation. Leukemia 15, 1471–1474 (2001). https://doi.org/10.1038/sj.leu.2402212

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.leu.2402212

Keywords

Search

Advanced search

Quick links