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Mutation study of the BCL10 gene in lymphoma with both RNA and DNA

Leukemia volume 15, pages 1139–1140 (2001)Cite this article

The BCL10 gene has been cloned from the chromosomal translocation t(1;14)(p22;q32) in a low-grade mucosa-associated tissue (MALT) lymphoma.1 The function of the protein is not yet clear, and it also remains to be proved whether BCL10 is an oncogene or a tumor suppressor gene.1,2 Willis et al1 suggested that BCL10 mRNA exhibited hypermutations in MALT lymphomas with t(1;14), as well as frequent mutations in 45% of B and T cell lineage lymphomas without 1q22 chromosomal rearrangements. In a recent issue of Leukemia, Luminari et al3 reported, however, that BCL10 mutations are rare, when they examined genomic DNA of various types of lymphoid malignancies. Some BCL10 gene abnormalities were proposed to be found only in RNA and not in genomic DNA,4,5 but no previous studies investigated DNA and RNA at the same time.4,5,6,7,8 To make the matter clear, we first analyzed the mutations of BCL10 RNA from 43 lymphomas, and for those lymphomas with RNA alterations we investigated the genomic DNA extracted from the same tumor and normal tissues from the same patients.

For the two follicular lymphoma patients with sequence alterations of BCL10 RNA we investigated the mutations of the DNA samples isolated from the same lymphoma tissue and bone marrow without documented lymphoma invasion by PCR and direct sequencing. In the patient with C/T heterozygosity, identical C/T heterozygosity was detected in both DNA from the same lymphoma sample and that from the bone marrow without lymphoma involvement, thus this single nucleotide substitution may probably be a polymorphism. In the lymphoma of this patient, which is considered to be monoclonal, both C485 and T485 alleles (according to numbering described by Willis et al1), appeared to be evenly expressed. This observation may indicate that BCL10 is biallelically expressed, as most genes are. In the patient with an 81 base pair deletion in RNA, the abnormal and normal alleles were detected evenly in the genomic DNA from the same tumor sample, but the mutated allele was not detected in his bone marrow without lymphoma (Figure 1b). Therefore, this deletion mutation is tumor-specific. Furthermore, as the RNA analysis of the tumor showed that more mutated allele was expressed than normal allele (Figure 1a), this mutant allele may predominantly be expressed in the lymphoma cells.

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References

  1. Willis TG, Jadayel DM, Du MQ, Peng H, Perry AR, Abdul-Rauf M, Price H, Karran L, Majekodunmi O, Wlodarska I, Pan L, Crook T, Hamoudi R, Isaacson PG, Dyner MJS . Bcl10 is involved in t(1;14)(p22;q32) of MALT B cell lymphoma and mutated in multiple tumor types Cell 1999 96: 35–45

    Article  CAS  Google Scholar 

  2. Zhang Q, Siebert R, Yan M, Hinzmann B, Cui X, Xue L, Rakestraw KM, Naeve CW, Beckmann G, Weisenburger DD, Sanger WG, Nowotny H, Callet-Bauchu E, Salles G, Dixit VM, Rosenthal A, Schlegelberger B, Morris SW . Inactivating mutations and overexpression of BCL10, a caspase recruitment domain-containing gene, in MALT lymphoma with t(1;14)(p22;q32) Nat Genet 1999 22: 63–68

    Article  CAS  Google Scholar 

  3. Luminari S, Intini D, Baldini L, Berti E, Bertoni F, Zucca E, Cro L, Maiolo AT, Cavalli F, Neri A . BCL10 gene mutations rarely occur in lymphoid malignancies Leukemia 2000 14: 905–908

    Article  CAS  Google Scholar 

  4. Fakruddin JM, Chaganti RS, Murty VV . Lack of BCL10 mutations in germ cell tumors and B cell lymphomas Cell 1999 97: 683–684; discussion 686–688

    Article  CAS  Google Scholar 

  5. Apostolou S, De Rienzo A, Murthy SS, Jhanwar SC, Testa JR . Absence of BCL10 mutations in human malignant mesothelioma Cell 1999 97: 684–686; discussion 686–688

    Article  CAS  Google Scholar 

  6. Takahashi H, Hosokawa Y, Suzuki R, Morishima Y, Nakamura S, Seto M . Infrequent BCL10 mutations in B-cell non-Hodgkin's lymphomas Jpn J Cancer Res 1999 90: 1316–1320

    Article  CAS  Google Scholar 

  7. Yuille MR, Stone JG, Bradshaw PS, Houlston RS . Bcl10 in chronic lymphocytic leukaemia and T-cell prolymphocytic leukaemia Br J Haematol 1999 107: 384–385

    Article  CAS  Google Scholar 

  8. Takahashi H, Maeda Y, Seto M, Hosokawa Y . Nucleotide insertions and deletions within the homopolymeric runs of adenines and thymidines of BCL10 cDNAs in normal peripheral bloodleukocytes Blood 2000 95: 2728–2729

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Clinical Research and Hematology, Nagoya National Hospital, Nagoya, Japan

    K Tsushita, H Ohashi, M Utsumi, M Shimoyama & H Nagai

  2. First Department of Internal Medicine, Nagoya University School of Medicine, Nagoya, Japan

    T Kinoshita

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Tsushita, K., Ohashi, H., Utsumi, M. et al. Mutation study of the BCL10 gene in lymphoma with both RNA and DNA. Leukemia 15, 1139–1140 (2001). https://doi.org/10.1038/sj.leu.2402154

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