Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Correspondence
  • Published:

Short-term growth potential of cryopreserved B chronic lymphocytic leukemia cells in suspension and in semisolid medium

Leukemia volume 15, pages 992–994 (2001)Cite this article

There has been speculation, but limited data, concerning the inferior survival of malignant cells compared with benign stem cells during cryopreservation.1,2 We have addressed this question by investigation of the proliferation and activation potential of freshly isolated and cryopreserved tumor cells from a cohort of 30 patients with typical B cell CLL and with blood lymphocyte counts above 30 × 109/l.

Peripheral blood mononuclear cells were isolated from heparinized (Noparin; Novo Nordik, Dagsvaerd, Denmark) blood samples by centrifugation over a Lymphoprep layer (Nycomed, Oslo, Norway) at a density of 1.077 g/ml as described elsewhere.3,4 Density gradient centrifugation yields, without other purification steps, were good (range 29–92%; mean 61.7%; s.d. 16.2%, n = 36). This information is necessary for interpretation of the results.5 The proportion of monocytes plus polyclonal T- and B-lymphocytes was 1–13%, indicating that 87–99% of the isolated cells represented the leukemic population. Immunophenotyping was performed by flow cytometry (FACSCalibur, Becton Dickinson Immunocytometry Systems, San Jose, CA, USA) using commercial mouse monoclonal antibodies and respective immunoglobulin isotype controls.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2
Figure 3

References

  1. Allieri M, Lopez M, Douay L, Mary J, NGuyen L, Gorin N . Clonogenic leukemic progenitor cells in acute myelocytic leukemia are highly sensitive to cryopreservation: possible purging effect for autologous bone marrow transplantation Bone Marrow Transplant 1991 7: 101–105

    CAS  PubMed  Google Scholar 

  2. Verdonck L, Heesbeen E, van Heuggten H, Staal G, Rijksen G . The cytotoxicity of alkyl-phospholipid on clonogenic leukemia cells and on normal bone marrow progenitor cells is highly, but differentially, increased by cryopreservation Bone Marrow Transplant 1992 9: 241–245

    CAS  PubMed  Google Scholar 

  3. Vilpo J, Vilpo L, Hurme M, Vuorinen P . Induction of beta-2-microglobulin release in vitro by chronic lymphocytic leukaemia cells: relation to total protein synthesis Leuk Res 1999 23: 913–920

    Article  CAS  Google Scholar 

  4. Larramendy M, Siitonen S, Hurme M, Vilpo L, Vilpo J, Knuutila S . Optimized mitogen stimulation induces proliferation of neoplastic B-cells in chronic lymphocytic leukemia: significance for cytogenetic analysis Cytogenet Cell Genet 1998 82: 215–221

    Article  CAS  Google Scholar 

  5. Vilpo J, Vilpo L, Hulkkonen J, Lankinen M, Kuusela P, Hurme M . Non-specific binding compromises the purification yields of leukemic B-cells in chronic lymphocytic leukemia: prevention by collagen coating Eur J Haematol 1998 60: 65–67

    Article  CAS  Google Scholar 

  6. Kipps T . Chronic lymphocytic leukemia Curr Opin Hematol 2000 7: 223–234

    Article  CAS  Google Scholar 

  7. Koski T, Karhu R, Vilpo L, Knuutila S, Vilpo J . Complex chromosomal aberrations in chronic lymphocytic leukemia are associated with cellular drug and irradiation resistance Eur J Haematol 2000 65: 32–39

    Article  CAS  Google Scholar 

  8. Dreger P, Michallet M, Schmitz N . Stem-cell transplantation for chronic lymphocytic leukemia: the 1999 perspective Ann Oncol 2000 11 (Suppl. 1): 49–53

    Article  Google Scholar 

Download references

Acknowledgements

This work was supported by grants from the Medical Research Fund of Tampere University Hospital and the Finnish Foundation for Cancer Research. We thank Leena Pankko and Merja Suoranta for their skilful technical assistance.

Author information

Authors and Affiliations

  1. Department of Clinical Chemistry, Laboratory of Molecular Hematology, Tampere University Hospital and University of Tampere Medical School, Tampere, Finland

    J Vilpo & L Vilpo

Authors
  1. J Vilpo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L Vilpo
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vilpo, J., Vilpo, L. Short-term growth potential of cryopreserved B chronic lymphocytic leukemia cells in suspension and in semisolid medium. Leukemia 15, 992–994 (2001). https://doi.org/10.1038/sj.leu.2402101

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.leu.2402101

This article is cited by

Search

Advanced search

Quick links