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Cell Biology

Somatostatin induces migration of acute myeloid leukemia cells via activation of somatostatin receptor subtype 2

Leukemia volume 15, pages 621–627 (2001)Cite this article

Abstract

Somatostatin, a neuropeptide with multiple activities, exerts its function via G-coupled membrane receptors. Five somatostatin receptor subtypes, sst1–5, have been identified. We have recently established that somatostatin acts as a chemoattractant on normal hematopoietic progenitor cells. Here, we studied the expression of somatostatin receptors (sst) on leukemic cells from 16 AML patients. Using fluorescent somatostatin (Fluo-SS) in flow cytometry, we found that sst are expressed in variable amounts on primary AML cells. Reverse transcriptase polymerase chain reaction (RT-PCR) analysis and immunochemistry revealed that only sst subtype 2 is expressed by AML cells. Using a two-chamber in vitro migration assay, we show that AML cells migrated towards a gradient of octreotide, a stable synthetic analogue of somatostatin. The degree of migration correlated with the cell surface density of sst2 as measured by Fluo-SS binding. These findings indicate that somatostatin influences trafficking of AML cells, which may have implications for the distribution of AML cells in the body and for clinical applications of somatostatin and analogues thereof in the context of AML.

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Acknowledgements

We thank Dr Alister C Ward and Dr Mirjam HA Hermans for critical reading of the manuscript and Dr A Schonbrunn for providing the R2–88 antibody. This work was supported by funds from the Nederlandse Wetenschappelijke Organisatie (NWO), and grants from the Dutch Cancer Society.

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Authors and Affiliations

  1. Institute of Hematology, Dr Daniel den Hoed Cancer Center, Rotterdam, The Netherlands

    SPMA Oomen, N Verplanke, B Löwenberg & IP Touw

  2. Department of Internal Medicine III, Erasmus University, Rotterdam, The Netherlands

    EGR Lichtenauer-Kaligis, J Hofland & SWJ Lamberts

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  1. SPMA Oomen
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Oomen, S., Lichtenauer-Kaligis, E., Verplanke, N. et al. Somatostatin induces migration of acute myeloid leukemia cells via activation of somatostatin receptor subtype 2. Leukemia 15, 621–627 (2001). https://doi.org/10.1038/sj.leu.2402061

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