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Lymphomas

Analysis of the immunoglobulin heavy chain gene variable region of CD5-positive and -negative diffuse large B cell lymphoma

Leukemia volume 15, pages 452–457 (2001)Cite this article

Abstract

We analyzed nucleotide sequence and intraclonal diversity of the rearranged immunoglobulin heavy chain gene variable region (VH gene) of CD5+ and CD5− diffuse large B cell lymphoma (DLBCL) to clarify the cell origin of de novo CD5+DLBCL. Ten cases of CD5+ DLBCL and 29 cases of CD5− DLBCL were analyzed. The frequencies of somatic mutation were 0.7 to 12.9% (average, 6.2%) in CD5+ DLBCL and 2.0 to 25.9% (average, 11.1%) in CD5− DLBCL. The ongoing mutation rate was estimated from the number of further single base-substitutions, expressed as a percentage of the total number of nucleotides in 10 cloned PCR products for each case (%). The averages of the ongoing mutation rate of CD5+ DLBCL (four cases) and CD5− DLBCL (seven cases) were 0.051% and 0.197%, respectively. The rate of CD5+ DLBCL was significantly lower than that of CD5− DLBCL (t-test, P = 0.024). These data may indicate that the cell origin of CD5+ DLBCL is different from that of CD5− DLBCL. CD5 is not an activated antigen in DLBCL, but a specific marker of the B1 subset of the B cells, and de novo CD5+ DLBCL may therefore be derived from this unique subset.

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Authors and Affiliations

  1. Department of Pathology, Fukushima Medical University School of Medicine, Fukushima, Japan

    N Nakamura, T Kuze, Y Hashimoto, Y Hara, S Hoshi, Y Sasaki, A Shirakawa, M Sato & M Abe

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  1. N Nakamura
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  2. T Kuze
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Nakamura, N., Kuze, T., Hashimoto, Y. et al. Analysis of the immunoglobulin heavy chain gene variable region of CD5-positive and -negative diffuse large B cell lymphoma. Leukemia 15, 452–457 (2001). https://doi.org/10.1038/sj.leu.2402031

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