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Clinical Laboratory Correlates: AML and p27kip1

Prognostic significance of the cell cycle inhibitor p27Kip1 in acute myeloid leukemia

Leukemia volume 14, pages 28–33 (2000)Cite this article

Abstract

There are few molecular biologic determinants that are prognostic for patients with acute myeloid leukemia (AML). Hence, we examined whether cellular levels of the cyclin-dependent kinase inhibitor p27Kip1 in acute myeloid leukemia could be used to predict clinical outcome in AML. Using immunoblot analysis, levels of p27 were assessed in blast cells from 72 AML patients who were registered and treated by the identical chemotherapy protocol. AML cases were classified into three groups on the basis of the percentage of the expression level of p27 compared to a control cell line. AML cases exhibiting p27 expression at low, moderate, and high levels were 43, 9, and 20 cases, respectively. No significant differences in the rates of complete remission (CR) were observed among the three groups. Although the level of p27 expression was not correlated with any other possible prognostic markers, such as age, white blood cell count, chromosome abnormalities, and FAB subclasses, patients with high p27 expression had a significantly increased disease-free survival (DFS) (78% vs 19%, P = 0.004). We further examined the expression of cyclin E at the protein level in all 72 AML cases. We observed a statistically significant correlation between a high cyclin E level and a high p27 level (P < 0.005). However, we failed to find any correlation between the rates of cr or dfs and cyclin e expression. The present study reveals that levels of p27 expression can be one of the useful prognostic molecular markers for aml.

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Acknowledgements

This work was supported in part by Grants-in-Aid for Cancer Research (No. 9-2) from the Japanese Ministry of Health and Welfare, Tokyo, Japan. The authors thank S Suzuki and C Wakamatsu for their technical assistance. We also think Y Makino for her editorial assistance.

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Authors and Affiliations

  1. First Department of Internal Medicine, Nagoya University School of Medicine, Nagoya, Japan

    T Yokozawa, M Towatari, H Iida, K Takeya ma, M Tanimoto & H Saito

  2. Department of Infectious Diseases, Nagoya University Hospital, Nagoya, Japan

    H Kiyoi & T Naoe

  3. Department of Hematology, Tokyo Women's Medical College, Tokyo, Japan

    T Motoji

  4. Second Department of Internal Medicine, Kumamoto University School of Medicine, Kumamoto, Japan

    N Asou

  5. Third Department of Internal Medicine, Dokkyo University School of Medicine, Tochigi, Japan

    K Saito

  6. Department of Medicine, National Sanatorium Minami Okayama Hospital, Okayama, Japan

    M Takeuchi

  7. Department of Medical Oncology, National Cancer Center Hospital, Tokyo, Japan

    Y Kobayashi

  8. Department of Medicine, Saiseikai Maebashi Hospital, Maebashi, Japan

    S Miyawaki

  9. Japan Red Cross Nagoya First Hospital, Nagoya, Japan

    Y Kodera

  10. Department of Medicine III, Hamamatsu University Medical School, Hamamatsu, Japan

    R Ohno

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Yokozawa, T., Towatari, M., Iida, H. et al. Prognostic significance of the cell cycle inhibitor p27Kip1 in acute myeloid leukemia. Leukemia 14, 28–33 (2000). https://doi.org/10.1038/sj.leu.2401640

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  • DOI: https://doi.org/10.1038/sj.leu.2401640

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