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Megakaryocytopoiesis

Establishment and characterization of a new human megakaryoblastic cell line (SET-2) that spontaneously matures to megakaryocytes and produces platelet-like particles

Leukemia volume 14, pages 142–152 (2000)Cite this article

Abstract

A new factor-independent megakaryoblastic cell line, designated SET-2, was established from the peripheral blood of a patient with leukemic transformation of essential thrombocythemia (ET). SET-2 expressed CD 4, 7, 13, 33, 34, 36, 38, 41, 61, 71, 117, 126, 130 and c-mpl. In addition, it spontaneously produced numerous platelet-like particles in liquid culture. These particles were shown to be the same size as normal platelets, and to express CD 36, 38, 41, 61 and 71. Proliferation of SET-2 was not influenced by thrombopoietin (TPO) and other hemopoietic cytokines. SET-2 was found to express the platelet-specific proteins such as platelet factor 4 and β-thromboglobulin. The levels of expression were not altered by TPO. SET-2 also secreted interleukin-6 into the supernatants, as well as normal megakaryocytes. These results suggest that SET-2 spontaneously matures to megakaryocytes and produces platelet-like particles. These findings indicate that SET-2 may be useful for investigating the proliferation and differentiation mechanisms of leukemia cells and the role of c-mpl on megakaryoblasts, megakaryocytes, and platelets in ET.

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Acknowledgements

We thank Dr K Ueno (Division of Persistent and Oncogenic Viruses, Center for Chronic Viral Diseases, Faculty of Medicine, Kagoshima University, Kagoshima, Japan) for his technical help in the detection of EB virus infection using EBER-1 ISH method, and Dr T Tanaka (Teijin Bio Laboratories, Tokyo, Japan) for the help provided in performing the DNA fingerprinting.

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  1. Second Department of Internal Medicine, Faculty of Medicine, Kagoshima University, Kagoshima, Japan

    K Uozumi, M Otsuka, N Ohno, T Moriya ma, S Suzuki, S Shimotakahara, S Hanada & T Ari ma

  2. Department of Hematology and Oncology, Osaka University Medical School, Osaka, Japan

    I Matsumura

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Uozumi, K., Otsuka, M., Ohno, N. et al. Establishment and characterization of a new human megakaryoblastic cell line (SET-2) that spontaneously matures to megakaryocytes and produces platelet-like particles. Leukemia 14, 142–152 (2000). https://doi.org/10.1038/sj.leu.2401608

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