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Flow cytometric detection of B cell abnormal maturation in chronic myeloid leukemia

Leukemia volume 14, pages 339–340 (2000)Cite this article

Chronic myeloid leukemia (CML) is a neoplastic disorder arising from a stem cell with multilineage differentiation capabilities. The typical translocation t(9;22) has been identified in different lineages using cytometric and molecular methods. In these studies, all the maturation stages of granulopoiesis, erythropoiesis and megakaryocytopoiesis showed the t(9;22).1 B cell involvement in the neoplastic process is more controversial despite the development of frequent lymphoid blast crisis in the terminal phases of the disease. Clonality search has been hampered by the low number of lymphocytes present in the blood and bone marrow of CML patients.2 Torlakovic et al3 found the Ph chromosome in CD20 lymphocytes in two patients with CML using tricolor immunophenotyping/FISH. CD3 lymphocytes were negative in all the patients tested. Haferlach et al2 have recently demonstrated the t(9;22) translocation in B and T lymphocytes in the chronic phase of CML.

Flow cytometry is an interesting approach to the study of the lymphoid compartment in CML. Lucio et al4 have analyzed 38 bone marrow samples from normal adult volunteers using the combination CD10 CD20 CD19 and have defined three major CD19 B cell maturation stages: CD20− CD19+ CD10+ is the most immature subpopulation which in adults represents 0.12 ± 0.12% of the total BM nucleated cells; CD20+ CD19+ CD10+ is the intermediate subpopulation which accounts for 0.8 ± 0.59%; and CD20+ CD19+ CD10−) is the most mature subpopulation (2.22 ± 0.99) of total BM nucleated cells.

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  1. Hematology Department, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain

    L Muñoz, M Bellido, J Sierra & JF Nomdedéu

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  1. L Muñoz
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  2. M Bellido
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  3. J Sierra
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  4. JF Nomdedéu
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Muñoz, L., Bellido, M., Sierra, J. et al. Flow cytometric detection of B cell abnormal maturation in chronic myeloid leukemia. Leukemia 14, 339–340 (2000). https://doi.org/10.1038/sj.leu.2401549

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