Analysis of the INK4a/ARF locus in non-Hodgkin’s lymphomas using two new internal microsatellite markers

Abstract

Inactivation of the INK4a/ARF locus is a frequent event in non-Hodgkin’s lymphomas (NHLs), which may be attributed to deletion, point mutation, and 5′ CpG methylation at its promoter region. In the present study we evaluated the occurrence of deletions and genetic instability of INK4a/ARF locus in 30 paired normal and tumor samples of B cell NHLs by conducting an allelotypic analysis with two new polymorphic markers, one located at the intron 1 of p16^INK4a gene and the other one placed downstream exon 1β of p19^ARF. Comparison of these results with those obtained in a previous paper using flanking markers (D9S171, D9S942, D9S958 and IFNA) allowed us to detect two new cases of microsatellite instability (L-446 and L-442), and to confirm the occurrence of LOH at the INK4a/ARF locus in one tumor (M-3770). On the contrary, this locus is not affected in three different tumors (L-421, L-272 and L-159) which exhibited LOH at some of the flanking markers.