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Clinical Laboratory Correlates

Internal tandem duplication of the FLT3 gene and clinical evaluation in childhood acute myeloid leukemia

Leukemia volume 13, pages 38–43 (1999)Cite this article

Abstract

We analyzed tandem duplication in the juxtamembrane (JM) domain of the FLT3 (FMS-like tyrosine kinase 3/FLK2, CD135) gene in 94 children with acute myeloid leukemia (AML) and evaluated its correlation with clinical features. Longer polymerase chain reaction (PCR) products were observed in five patients; 1/3 of M0, 1/9 of M1, 1/39 of M2, 1/9 of M3 and 1/12 of M5. The sequence analyses of abnormal PCR products showed that all the abnormal products were derived from tandem duplications involving the JM domain and that all the lengthened sequences were in-frame as we previously reported. Statistical analyses revealed a significantly lower incidence of the tandem duplication in childhood AML patients than in adult patients (P < 0.05), and significantly shorter disease-free survival in patients with mutant FLT3 than in patients with wild-type FLT3 (P < 0.05). our results suggest that the tandem duplication in the jm domain of the FLT3 gene is not a frequent phenomenon but might be a factor of poor prognosis in childhood patients with AML.

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Authors and Affiliations

  1. Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, Japan

    T Iwai, S Yokota, M Nakao, T Okamoto & M Taniwaki

  2. Pediatrics, Iwate Prefectural Kitakami Hospital, Japan

    N Onodera

  3. Pediatrics, Akita University School of Medicine, Japan

    A Watanabe

  4. Pediatrics, Fukushima Medical School, Japan

    A Kikuta

  5. Pediatrics, Niigata University of School of Medicine, Japan

    A Tanaka

  6. Pediatrics, Niigata Cancer Center Hospital, Japan

    K Asami

  7. Pediatrics, National Defense Medical College, Japan

    I Sekine

  8. Pediatrics, Nihon University School of Medicine, Japan

    H Mugishima

  9. Pediatrics, Kanazawa University School of Medicine, Japan

    Y Nishimura & S Koizumi

  10. Division of Hematology and Oncology, Shizuoka Children’s Hospital, Japan

    Y Horikoshi & J Mimaya

  11. Pediatrics, Shiga University of Medical Science, Japan

    S Ohta

  12. Pediatrics, Faculty of Medicine, Tottori University, Japan

    K Nishikawa

  13. National Kagawa Children’s Hospital, Japan

    A Iwai

  14. Pediatrics, Shikoku Cancer Center Hospital, Japan

    T Shimokawa

  15. Pediatrics, Nagasaki University, School of Medicine, Japan

    M Nakayama

  16. Pediatrics, Faculty of Medicine, Kagoshima University, Japan

    K Kawakami

  17. Pediatrics, University of The Ryukyu, School of Medicine, Japan

    T Gushiken & N Hyakuna

  18. Pediatrics, Aichi Medical University, Japan

    N Katano, M Tsurusawa & T Fujimoto

Authors
  1. T Iwai
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  2. S Yokota
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  3. M Nakao
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  4. T Okamoto
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  5. M Taniwaki
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  6. N Onodera
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  7. A Watanabe
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  8. A Kikuta
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  9. A Tanaka
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  10. K Asami
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  11. I Sekine
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  12. H Mugishima
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  13. Y Nishimura
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  14. S Koizumi
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  15. Y Horikoshi
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  16. J Mimaya
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  17. S Ohta
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  18. K Nishikawa
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  19. A Iwai
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  20. T Shimokawa
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  21. M Nakayama
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  22. K Kawakami
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  23. T Gushiken
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  24. N Hyakuna
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  25. N Katano
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  26. M Tsurusawa
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  27. T Fujimoto
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Iwai, T., Yokota, S., Nakao, M. et al. Internal tandem duplication of the FLT3 gene and clinical evaluation in childhood acute myeloid leukemia. Leukemia 13, 38–43 (1999). https://doi.org/10.1038/sj.leu.2401241

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  • DOI: https://doi.org/10.1038/sj.leu.2401241

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