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Nucleotide Sequence of the Gene Coding for the Bacteriophage MS2 Coat Protein

Abstract

By characterization of fragments, isolated from a nuclease digest of MS2 RNA, the entire nucleotide sequence of the coat gene was established. A “flower”-like model is proposed for the secondary structure. The genetic code makes use of 49 different codons to specify the sequence of the 129 amino-acids long coat polypeptide.

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References

  1. Cold Spring Harbor Symp. Quant. Biol., 34, The Mechanism of Protein Synthesis (1969).

  2. Fiers, W., Lepoutre, L., and Vandendriessche, L., J. Mol. Biol., 13, 432 (1965).

    Article  CAS  Google Scholar 

  3. Min Jou, W., Hindley, J., and Fiers, W., Arch. Intern. Physiol. Biochem., 76, 194 (1968).

    Google Scholar 

  4. Adams, J. M., Jeppesen, P. G. N., Sanger, F., and Barrell, B. G., Nature, 223, 1009 (1969).

    Article  ADS  CAS  Google Scholar 

  5. Steitz, J. A., Nature, 224, 957 (1969).

    Article  ADS  CAS  Google Scholar 

  6. Nichols, J. L., Nature, 225, 147 (1970).

    Article  ADS  CAS  Google Scholar 

  7. Jeppesen, P. G. N., Nichols, J. L., Sanger, F., and Barrell, B. G., Cold Spring Harbor Symp. Quant. Biol., 35, 13 (1970).

    Article  CAS  Google Scholar 

  8. Min Jou, W., Haegeman, G., and Fiers, W., FEBS Lett., 13, 105 (1971).

    Article  CAS  Google Scholar 

  9. Fiers, W., Contreras, R., De Wachter, R., Haegeman, G., Merregaert, J., Min Jou, W., and Vandenberghe, A., Biochimie, 53, 495 (1971).

    Article  CAS  Google Scholar 

  10. De Wachter, R., and Fiers, W., Methods in Enzymology (edit. by Colowick, S. P., and Kaplan, N. O.), 21, 167 (Academic Press, New York and London, 1971).

    Google Scholar 

  11. De Wachter, R., Merregaert, J., Vandenberghe, A., Contreras, R., and Fiers, W., Europ. J. Biochem., 22, 400 (1971).

    Article  CAS  Google Scholar 

  12. Weber, K., and Konigsberg, W., J. Biol. Chem., 242, 3563 (1967).

    CAS  Google Scholar 

  13. Weber, K., Biochemistry, 6, 3144 (1967).

    Article  CAS  Google Scholar 

  14. Lin, J. Y., Tsung, C. M., and Fraenkel-Conrat, J., J. Mol. Biol., 24, 1 (1967).

    Article  CAS  Google Scholar 

  15. Vandekerckhove, J., Francq, H., and Van Montagu, M., Arch. Intern. Physiol. Biochim., 77, 175 (1969).

    CAS  Google Scholar 

  16. Nichols, J. L., and Robertson, H. D., Biochim. Biophys. Acta 228, 676 (1971).

    Article  CAS  Google Scholar 

  17. De Wachter, R., and Fiers, W., Analyt. Biochem. (in the press).

  18. Brownlee, G. G., and Sanger, F., Europ. J. Biochem., 11, 395 (1969).

    Article  CAS  Google Scholar 

  19. Sanger, F., Brownlee, G. G., and Barrell, B. G., J. Mol. Biol., 13, 337 (1965).

    Article  Google Scholar 

  20. Brownlee, G. G., and Sanger, F., J. Mol. Biol., 23, 337 (1967).

    Article  CAS  Google Scholar 

  21. Scott, D. W., Virology, 26, 85 (1965).

    Article  CAS  Google Scholar 

  22. Strauss, J. H., and Sinsheimer, R. L., J. Mol. Biol., 7, 43 (1963).

    Article  CAS  Google Scholar 

  23. Gesteland, R. F., and Boedtker, H., J. Mol. Biol., 8, 496 (1964).

    Article  CAS  Google Scholar 

  24. Mitra, S., Enger, M. D., and Kaesberg, P., Proc. US Nat. Acad. Sci., 50, 68 (1963).

    Article  ADS  CAS  Google Scholar 

  25. Boedtker, H., Biochemistry, 6, 2718 (1967).

    Article  CAS  Google Scholar 

  26. Isenberg, H., Cotter, R. I., and Gratzer, W. B., Biochim. Biophys. Acta, 232, 184 (1971).

    Article  CAS  Google Scholar 

  27. McPhie, P., Hounsell, J., and Gratzer, W. B., Biochemistry, 5, 988 (1966).

    Article  CAS  Google Scholar 

  28. Gould, H. J., Biochemistry, 5, 1103 (1966).

    Article  CAS  Google Scholar 

  29. Adams, A., Lindahl, T., and Fresco, J. R., Proc. US Nat. Acad. Sci., 57, 1684 (1967).

    Article  ADS  CAS  Google Scholar 

  30. Strauss, J. H., and Sinsheimer, R. L., J. Mol. Biol., 34, 453 (1968).

    Article  CAS  Google Scholar 

  31. Fukami, H., and Imahori, K., Proc. US Nat. Acad. Sci., 68, 570 (1971).

    Article  ADS  CAS  Google Scholar 

  32. Richards, E. G., Coll, J. A., and Gratzer, W. B., Analyt. Biochem., 12, 452 (1965).

    Article  CAS  Google Scholar 

  33. Bishop, D. H. L., Claybrook, J. R., and Spiegelman, S., J. Mol. Biol., 26, 373 (1967).

    Article  CAS  Google Scholar 

  34. Tinoco, I., Uhlenbeck, O. C., and Levine, M. D., Nature, 230, 362 (1971).

    Article  ADS  CAS  Google Scholar 

  35. Cory, S., Spahr, P. F., and Adams, J. M., Cold Spring Harbor Symp. Quant. Biol., 35, 1 (1970).

    Article  CAS  Google Scholar 

  36. Gupta, S. L., Chen, J., Schaefer, L., Lengyel, P., and Weissman, S. M., Biochem. Biophys. Res. Commun., 39, 883 (1970).

    Article  CAS  Google Scholar 

  37. Lodish, H. F., and Zinder, N. D., J. Mol. Biol., 19, 333 (1966).

    Article  CAS  Google Scholar 

  38. Gussin, G. N., J. Mol. Biol., 21, 435 (1966).

    Article  CAS  Google Scholar 

  39. Engelhardt, D. L., Webster, R. E., and Zinder, N. D., J. Mol. Biol., 29, 45 (1967).

    Article  CAS  Google Scholar 

  40. Roberts, J. W., and Gussin, G. N., J. Mol. Biol., 30, 565 (1967).

    Article  CAS  Google Scholar 

  41. Zinder, N. D., and Cooper, S., Virology, 23, 152 (1964).

    Article  CAS  Google Scholar 

  42. Webster, R. E., Engelhardt, D. L., Zinder, N. D., and Konigsberg, W., J. Mol. Biol., 29, 27 (1967).

    Article  CAS  Google Scholar 

  43. Tooze, J., and Weber, K., J. Mol. Biol., 28, 311 (1967).

    Article  CAS  Google Scholar 

  44. Van Montagu, M., Arch. Intern. Physiol. Biochim., 74, 941 (1966).

    CAS  Google Scholar 

  45. Fiers, W., Van Montagu, M., De Wachter, R., Haegeman, G., Min Jou, W., Messens, E., Remaut, E., Vandenberghe, A., and Van Styvendaele, B., Cold Spring Harbor Symp. Quant. Biol., 34, 697 (1969).

    Article  CAS  Google Scholar 

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JOU, W., HAEGEMAN, G., YSEBAERT, M. et al. Nucleotide Sequence of the Gene Coding for the Bacteriophage MS2 Coat Protein. Nature 237, 82–88 (1972). https://doi.org/10.1038/237082a0

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  • DOI: https://doi.org/10.1038/237082a0

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