Abstract
WE report here the isolation from messenger-free 80S wheat embryo ribosomes of 40S and 60S ribosomal subunits active in protein synthesis directed by natural messenger RNA (Table 1). This has enabled us to provide firm biochemical evidence satisfying the hypothesis that initiation in eukaryotic cells proceeds through the formation of a 40S ribosomal subunit-mRNA-Met-tRNA complex (complex I) which subsequently combines with a 60S ribosomal subunit to form an 80S monoribosome (complex II) functional in protein synthesis.
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WEEKS, D., VERMA, D., SEAL, S. et al. Role of Ribosomal Subunits in Eukaryotic Protein Chain Initiation. Nature 236, 167–168 (1972). https://doi.org/10.1038/236167a0
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DOI: https://doi.org/10.1038/236167a0
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