Letter | Published:

Spin Echo Studies on Cellular Water

Abstract

HIGH resolution nuclear magnetic resonance (NMR) studies on rat skeletal muscle have recently shown that cellular water produces an absorption line almost ten times as broad as the line width of pure water1. This suggests that these water molecules are at least partly bounded such that the proton correlation time is increased. Similar observations have also been made for deuterated water in skeletal muscle2 and for H2O in other tissues3–5. Our results further substantiate the notion that ordered water exists in biological tissue and rules out several criticisms of this interpretation.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Hazlewood, C. F., Nichols, B. L., and Chamberlain, N. F., Nature, 222, 747 (1969).

  2. 2

    Cope, F. W., Biophys. J., 9, 303 (1969).

  3. 3

    Bratton, C. B., Hopkins, A. L., and Weinberg, J. W., Science, 147, 738 (1968).

  4. 4

    Abetsedarskaya, L. A., Miftakhutdinova, F. G., and Fedorov, V. D., Biofizika, 13, 630 (1968); translation in Biophysics, 13, 750 (1968).

  5. 5

    Swift, T. J., and Fritz, O. G., Biophys. J., 9, 54 (1969).

  6. 6

    Carr, H. Y., and Purcell, E. M., Phys. Rev., 94, 630 (1954).

  7. 7

    Hazlewood, C. F., Nichols, B. L., Chang, D. C., and Brown, B., Johns Hopkins Med. J., 128, 117 (1971).

  8. 8

    Meiboom, S., Luz, Z., and Gill, D., J. Chem. Phys., 27, 1411 (1957).

  9. 9

    Wang, J. H., J. Chem. Phys., 69, 4412 (1965).

  10. 10

    Hansen, J. R., and Lawson, K. D., Nature, 225, 542 (1970).

  11. 11

    Woessner, D. E., J. Phys. Chem., 67, 1365 (1963).

  12. 12

    Wayne, R. C., and Cotts, R. M., Phys. Rev., 151, 264 (1966).

  13. 13

    Robertson, B., Phys. Rev., 151, 273 (1966).

  14. 14

    Glasel, J. A., Nature, 227, 704 (1970).

  15. 15

    Hori, T., Teion Kagaku, Butsuri (Low Temp. Science), 15, 34 (1956). English Translation No. 62, US Army Snow, Ice and Permafrost Establishment, Wilmette, Illinois.

  16. 16

    Frank, H. S., and Wen, W. Y., Discussions Faraday Soc., 24, 133 (1957).

  17. 17

    Horne, R. A., Day, A. F., Young, R. P., and Yu, N. T., Electrochimica Acta, 13, 397 (1968).

  18. 18

    Schultz, R. D., and Asunmaa, S. K., Recent Progress in Surface Science, 3, 291 (Academic Press, New York, 1970).

  19. 19

    Drost-Hansen, W., Ind. Eng. Chem., 61, 10 (1969).

  20. 20

    Drost-Hansen, W., in Chemistry of the Cell Interface (edit. by Brown, H. D.) (Academic Press, New York, in the press).

  21. 21

    Grant, E. H., Ann. NY Acad. Sci., 125, 418 (1965).

  22. 22

    Hinke, J. A. M., J. Gen. Physiol., 56, 521 (1970).

  23. 23

    Ling, G. N., and Negendank, W., Physiol. Chem. and Phys., 2, 15 (1970).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.