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Is a Specific Protein Responsible for the Supercoiling of Polyoma DNA?


POLYOMA and SV40 DNAs contain enough genetic information to code for half a dozen proteins. Very little is known about these proteins, although their recognition is of great interest in view of the oncogenic potential of the genomes. Recently, however, polyoma DNA from productively infected cells was isolated in the form of a protein–DNA complex distinct from the virus1 (our work in preparation). In an attempt to explore the nature and function of its protein moiety, we have investigated the effect of various inhibitors of protein synthesis on the formation and properties of this complex. We report here the results we obtained with puromycin.

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