Ligandin: a Hepatic Protein which Binds Steroids, Bilirubin, Carcinogens and a Number of Exogenous Organic Anions

Abstract

THREE protein preparations from the rat liver 100,000g supernatant fraction have been independently purified to homogeneity. They are basic azodye carcinogen-binding protein (β-ABP)1,2, corticosteroid Binder I3,4 and Y protein5. The compounds they bind include certain steroids and their metabolites, bilirubin and certain carcinogens and their metabolites. Several dyes and cholecystographic agents and other organic anions are also bound (Table 1). Binding occurs whether the compounds are injected in vivo or added in vitro to liver homogenates.

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References

  1. 1

    Ketterer, B., and Whitehead, J. K., FEBS Abstracts, 57 (1965).

  2. 2

    Ketterer, B., Ross-Mansell, P., and Whitehead, J. K., Biochem. J., 103, 316 (1967).

    CAS  Article  Google Scholar 

  3. 3

    Morey, K. S., and Litwack, G., Biochemistry, 8, 4813 (1969).

    CAS  Article  Google Scholar 

  4. 4

    Singer, S., Morey, K. S., and Litwack, G., Physiol. Chem. Physics, 2, 117 (1970).

    CAS  Google Scholar 

  5. 5

    Levi, A. J., Gatmaitan, Z., and Arias, I. M., J. Clin. Invest., 48, 2156 (1969).

    CAS  Article  Google Scholar 

  6. 6

    Ketterer, B., and Beale, D., Biochem. J., 122, 53p (1971).

    CAS  PubMed  PubMed Central  Google Scholar 

  7. 7

    Litwack, G., and Morey, K. S., Biochem. Biophys. Res. Commun., 38, 1141 (1970).

    CAS  Article  Google Scholar 

  8. 8

    Fleischner, G., Mishkin, S., Reyes, H., Robbins, J., Levi, A. J., Gatmaitan, Z., and Arias, I. M., J. Clin. Invest., 50, 31a (1971).

    Google Scholar 

  9. 9

    Litwack, G., Morey, K. S., and Ketterer, B., in Drugs and Cellular Control Mechanisms (edit. by Rabin, B. R.) (Macmillan, London, in the press).

  10. 10

    Levi, A. J., Gatmaitan, Z., and Arias, I. M., Lancet, ii, 139 (1969).

  11. 11

    Fleischner, G., and Arias, I. M., Amer. J. Med., 48, 576 (1970).

    Article  Google Scholar 

  12. 12

    Levi, A. J., Gatmaitan, Z., and Arias, I. M., New Engl. J. Med., 283, 1136 (1970).

    CAS  Article  Google Scholar 

  13. 13

    Singer, S., and Litwack, G., Endocrinology, 88, 1448 (1971)

    CAS  Article  Google Scholar 

  14. 14

    Levine, R., Reyes, H., Levi, A. J., Gatmaitan, Z., and Arias, I. M., Nature New Biology, 231, 277 (1971).

    CAS  Article  Google Scholar 

  15. 15

    Baldwin, R. W., Barker, C. R., and Moore, M., Brit. J. Cancer, 22, 776 (1968).

    CAS  Article  Google Scholar 

  16. 16

    Singer, S., and Litwack, G., Cancer Res., 31, 1364 (1971).

    CAS  PubMed  Google Scholar 

  17. 17

    Ketterer, B., and Christodoulides, L., Chem.-Biol. Interact., 1, 173 (1969).

    CAS  Article  Google Scholar 

  18. 18

    Reyes, H., Levi, A. J., Gatmaitan, Z., and Arias, I. M., Proc. US Nat. Acad. Sci., 64, 168 (1969).

    ADS  CAS  Article  Google Scholar 

  19. 19

    Reyes, H., Levi, A. J., Levine, R., Gatmaitan, Z., and Arias, I. M., Proc. New York Acad. Sci. (in the press).

  20. 20

    Reyes, H., Levi, A. J., Gatmaitan, Z., and Arias, I. M., J. Clin. Invest. (in the press).

  21. 21

    Bakay, B., and Sorof, S., Cancer Res., 24, 1814 (1964).

    CAS  Google Scholar 

  22. 22

    Ketterer, B., Beale, D., Litwack, G., and Hackney, J. F., Chem.-Biol. Interact., 3, 285 (1971).

    CAS  Article  Google Scholar 

  23. 23

    Litwack, G., and Singer, S., in Biochemical Actions of Hormones (edit. by Litwack, G.), 2 (Academic Press, New York) (in the press).

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LITWACK, G., KETTERER, B. & ARIAS, I. Ligandin: a Hepatic Protein which Binds Steroids, Bilirubin, Carcinogens and a Number of Exogenous Organic Anions. Nature 234, 466–467 (1971). https://doi.org/10.1038/234466a0

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