Abstract
ALTHOUGH the hormone ecdysone is known to initiate moulting and metamorphosis in insects its precise mechanism and site of action are unclear. When injected into larvae of Chironomus tentans it stimulated the formation of specific puffs in the polytene, salivary gland chromosomes1, and two mechanisms have been proposed to explain this. Karlson and Sekeris2 postulated that the hormone acts directly on the genome to “derepress” it, allowing the DNA to be transcribed. Kroeger3–5, however, suggested that ecdysone activates a sodium pump at the cell membrane. We have now investigated by autoradiography the distribution of 3H-β-ecdysone in isolated salivary gland cells of D. virilis.
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CLAYCOMB, W., LAFOND, R. & VILLEE, C. Autoradiographic Localization of 3H-β-Ecdysone in Salivary Gland Cells of Drosophila virilis. Nature 234, 302–304 (1971). https://doi.org/10.1038/234302a0
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DOI: https://doi.org/10.1038/234302a0
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