Abstract
Injected frog eggs and oocytes provide a very sensitive assay system for the identification of messenger RNA and permit the study of translational control in living cells. The translation of each haemoglobin messenger RNA molecule once every 5–10 minutes for at least 24 hours makes it possible to recognize less than 1 ng of this messenger RNA.
This is a preview of subscription content, access via your institution
Relevant articles
Open Access articles citing this article.
-
mRNA-based therapeutics: powerful and versatile tools to combat diseases
Signal Transduction and Targeted Therapy Open Access 21 May 2022
-
Rhinella marina oocytes: a suitable alternative expression system for functional characterization of aquaglyceroporins
Scientific Reports Open Access 10 January 2019
-
mRNA as novel technology for passive immunotherapy
Cellular and Molecular Life Sciences Open Access 17 October 2018
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Rent or buy this article
Get just this article for as long as you need it
$39.95
Prices may be subject to local taxes which are calculated during checkout
References
Chantrenne, H., Burny, A., and Marbaix, G., Prog. Nucleic Acid Res. and Mol. Biol., 7, 173 (1967).
Gurdon, J. B., J. Embryol. Exp. Morphol., 20, 401 (1968).
Gurdon, J. B., Quart. J. Microsc. Sci., 101, 299 (1960).
Smith, L. D., and Ecker, R. E., Current Topics in Devel. Biol., 5, 1 (1970).
Kutsky, P. B., Eakin, R. M., Berg, W. E., and Kavanau, J. L., J. Exp. Zool., 124, 263 (1953).
Ecker, R. E., and Smith, L. D., Develop. Biol., 18, 232 (1968).
Stavnezer, J., and Huang, R.-C. C., Nature, 230, 172 (1971).
Labrie, F., Nature, 221, 1217 (1969).
Lingrel, J. B., in Methods in Protein Synthesis (edit. by Laskin, A. E., and Last, J. A.) (Marcel Dekker, New York, in the press).
Hunt, T., Hunter, T., and Munro, A., J. Mol. Biol., 43, 123 (1969).
Armentrout, S. A., Schinkel, R. D., and Simmons, L. R., Arch. Biochem. Biophys., 112, 304 (1965).
Lane, C. D., Marbaix, G., and Gurdon, J. B., J. Mol. Biol. (in the press).
Moar, V. A., Gurdon, J. B., and Marbaix, G., J. Mol. Biol. (in the press).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
GURDON, J., LANE, C., WOODLAND, H. et al. Use of Frog Eggs and Oocytes for the Study of Messenger RNA and its Translation in Living Cells. Nature 233, 177–182 (1971). https://doi.org/10.1038/233177a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/233177a0
This article is cited by
-
mRNA-based therapeutics: powerful and versatile tools to combat diseases
Signal Transduction and Targeted Therapy (2022)
-
Rhinella marina oocytes: a suitable alternative expression system for functional characterization of aquaglyceroporins
Scientific Reports (2019)
-
mRNA as novel technology for passive immunotherapy
Cellular and Molecular Life Sciences (2019)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.