Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Use of Frog Eggs and Oocytes for the Study of Messenger RNA and its Translation in Living Cells

Abstract

Injected frog eggs and oocytes provide a very sensitive assay system for the identification of messenger RNA and permit the study of translational control in living cells. The translation of each haemoglobin messenger RNA molecule once every 5–10 minutes for at least 24 hours makes it possible to recognize less than 1 ng of this messenger RNA.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy article

Get time limited or full article access on ReadCube.

$32.00

All prices are NET prices.

References

  1. Chantrenne, H., Burny, A., and Marbaix, G., Prog. Nucleic Acid Res. and Mol. Biol., 7, 173 (1967).

    Article  CAS  Google Scholar 

  2. Gurdon, J. B., J. Embryol. Exp. Morphol., 20, 401 (1968).

    CAS  PubMed  Google Scholar 

  3. Gurdon, J. B., Quart. J. Microsc. Sci., 101, 299 (1960).

    Google Scholar 

  4. Smith, L. D., and Ecker, R. E., Current Topics in Devel. Biol., 5, 1 (1970).

    Article  Google Scholar 

  5. Kutsky, P. B., Eakin, R. M., Berg, W. E., and Kavanau, J. L., J. Exp. Zool., 124, 263 (1953).

    Article  Google Scholar 

  6. Ecker, R. E., and Smith, L. D., Develop. Biol., 18, 232 (1968).

    Article  CAS  Google Scholar 

  7. Stavnezer, J., and Huang, R.-C. C., Nature, 230, 172 (1971).

    Article  ADS  CAS  Google Scholar 

  8. Labrie, F., Nature, 221, 1217 (1969).

    Article  ADS  CAS  Google Scholar 

  9. Lingrel, J. B., in Methods in Protein Synthesis (edit. by Laskin, A. E., and Last, J. A.) (Marcel Dekker, New York, in the press).

  10. Hunt, T., Hunter, T., and Munro, A., J. Mol. Biol., 43, 123 (1969).

    Article  CAS  Google Scholar 

  11. Armentrout, S. A., Schinkel, R. D., and Simmons, L. R., Arch. Biochem. Biophys., 112, 304 (1965).

    Article  CAS  Google Scholar 

  12. Lane, C. D., Marbaix, G., and Gurdon, J. B., J. Mol. Biol. (in the press).

  13. Moar, V. A., Gurdon, J. B., and Marbaix, G., J. Mol. Biol. (in the press).

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

GURDON, J., LANE, C., WOODLAND, H. et al. Use of Frog Eggs and Oocytes for the Study of Messenger RNA and its Translation in Living Cells. Nature 233, 177–182 (1971). https://doi.org/10.1038/233177a0

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1038/233177a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing