Abstract
DURING a series of experiments at these laboratories to compare the protective antigenicity of several possible vaccine strains of Cl. chauvoei, one strain proved to be outstanding in protecting against a standard highly virulent challenge strain1. We have reported that guinea-pigs protected by this strain against the challenge strain show only H (or flagella) but not O agglutinating antibodies against the challenge strain. Subsequent experiments (to be published) also showed an apparent association between the H antigen and the protective antigen of this strain. One of several methods chosen to investigate this apparent association was to attempt the preparation of a pure suspension of flagella from this strain for testing as a protective antigen.
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References
Chandler, H. M., and Gulasekharam, J., Austral. J. Exp. Biol. Med. Sci., 48, 187 (1910).
Joys, T. M., Antonie van Leeuwenhoek J. Microbiol. Serol., 34, 205 (1968).
Iino, T., Bact. Rev., 33, 454 (1969).
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CHANDLER, H., GULASEKHARAM, J. Removal and Separation of Flagella from Cells of Clostridium chauvoei and Preparation of a Pure Flagella Suspension. Nature 230, 121–122 (1971). https://doi.org/10.1038/230121a0
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DOI: https://doi.org/10.1038/230121a0
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