Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Removal and Separation of Flagella from Cells of Clostridium chauvoei and Preparation of a Pure Flagella Suspension

Abstract

DURING a series of experiments at these laboratories to compare the protective antigenicity of several possible vaccine strains of Cl. chauvoei, one strain proved to be outstanding in protecting against a standard highly virulent challenge strain1. We have reported that guinea-pigs protected by this strain against the challenge strain show only H (or flagella) but not O agglutinating antibodies against the challenge strain. Subsequent experiments (to be published) also showed an apparent association between the H antigen and the protective antigen of this strain. One of several methods chosen to investigate this apparent association was to attempt the preparation of a pure suspension of flagella from this strain for testing as a protective antigen.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Chandler, H. M., and Gulasekharam, J., Austral. J. Exp. Biol. Med. Sci., 48, 187 (1910).

    Article  Google Scholar 

  2. Joys, T. M., Antonie van Leeuwenhoek J. Microbiol. Serol., 34, 205 (1968).

    Article  CAS  Google Scholar 

  3. Iino, T., Bact. Rev., 33, 454 (1969).

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

CHANDLER, H., GULASEKHARAM, J. Removal and Separation of Flagella from Cells of Clostridium chauvoei and Preparation of a Pure Flagella Suspension. Nature 230, 121–122 (1971). https://doi.org/10.1038/230121a0

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1038/230121a0

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing