Abstract
DURING the determination by Edman degradation of the amino-acid sequence of small peptides and the C-terminal region of longer peptides, extensive losses of material can occur when the 2-methylamino-5-thiazolinone obtained from the N-terminal amino-acid is extracted from the remaining peptide. This problem is encountered in degradations in which the thiohydantoin is identified directly as well as in the subtractive Edman degradation2,3. In the latter procedure the amino-acids in the peptide are determined before and after removal of the N-terminal residue; the identity of the N-terminal amino-acid is established by difference. Because a separate peptide hydrolysis step and amino-acid analysis is required for each amino-acid in the sequence, the subtractive procedure is also relatively time consuming.
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References
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VANCE, D., FEINGOLD, D. Additive Edman Degradation to sequence Small Peptides. Nature 229, 121–123 (1971). https://doi.org/10.1038/229121a0
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DOI: https://doi.org/10.1038/229121a0
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