Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

New RNA Polymerase from Escherichia coli infected with Bacteriophage T7

Abstract

A new T7-specific RNA polymerase is found in T7 phage-infected cells. It is the product of T7 gene I and is physically and biochemically distinct from the host cell RNA polymerase. The synthesis of T7 RNA polymerase provides a direct explanation for the pleiotropic control of late T7 functions exerted by gene I.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Geiduschek, E. P., and Haselkorn, R., Ann. Rev. Biochem., 38, 647 (1969).

    Article  CAS  PubMed  Google Scholar 

  2. Calendar, R., Ann. Rev. Microbiol., 24 (in the press).

  3. Travers, A. A., Nature, 223, 1107 (1969); 225, 1009 (1970).

    Article  ADS  CAS  PubMed  Google Scholar 

  4. Summers, W. C., and Siegel, R. B., Nature, 223, 1111 (1969).

    Article  ADS  CAS  PubMed  Google Scholar 

  5. Symons, R. H., Biochim. Biophys. Acta, 155, 609 (1968).

    Article  CAS  PubMed  Google Scholar 

  6. Thomas, jun., C. A., and Abelson, J., in Proc. Nucleic Acid Res. (edit. by Cantoni, G. L., and Davies, D. R.), 553 (Harper and Row, New York, 1966).

    Google Scholar 

  7. Schachman, H. K., Adler, J., Radding, C. M., Lehman, I. R., and Kornberg, A., J. Biol. Chem., 235, 3242 (1960).

    CAS  PubMed  Google Scholar 

  8. Radding, C. M., Josse, J., and Kornberg, A., J. Biol. Chem., 237, 2869 (1962).

    CAS  PubMed  Google Scholar 

  9. Shapiro, A. L., Viñuela, E., and Maizel, jun., J. V., Biochem. Biophys. Res. Commun., 28, 815 (1967).

    Article  CAS  PubMed  Google Scholar 

  10. Berg, D., and Chamberlin, M., Biochemistry (in the press).

  11. Berg, D., Barrett, K., and Chamberlin, M., Methods Enzymol., 13 (in the press).

  12. Studier, F. W., Virology, 39, 562 (1969).

    Article  CAS  PubMed  Google Scholar 

  13. Englander, S. W., and Crowe, D., Anal. Biochem., 12, 579 (1965).

    Article  CAS  PubMed  Google Scholar 

  14. Richardson, C. C., and Kornberg, A., J. Biol. Chem., 239, 242 (1964).

    CAS  PubMed  Google Scholar 

  15. Burgess, R. R., J. Biol. Chem., 244, 6168 (1969).

    CAS  PubMed  Google Scholar 

  16. Wehrli, W., Nüesch, J., Knüsel, F., and Staehelin, M., Biochim. Biophys. Acta, 157, 215 (1968).

    Article  CAS  PubMed  Google Scholar 

  17. Tocchini-Valentini, G., Marino, P., and Colvill, A. J., Nature, 220, 275 (1968).

    Article  ADS  CAS  PubMed  Google Scholar 

  18. Siddhikol, C., Erbstoeszer, J., and Weisblum, B., J. Bact., 99, 151 (1969).

    CAS  PubMed  PubMed Central  Google Scholar 

  19. Chamberlin, M., and Berg, P., Proc. US Nat. Acad. Sci., 48, 81 (1962).

    Article  ADS  CAS  Google Scholar 

  20. Summers, W. C., and Szybalski, W., Biochim. Biophys. Acta, 166, 371 (1968).

    Article  CAS  PubMed  Google Scholar 

  21. Summers, W. C., and Szybalski, W., Virology, 34, 9 (1968).

    Article  CAS  PubMed  Google Scholar 

  22. Martin, R. G., and Ames, B. N., J. Biol. Chem., 236, 1372 (1961).

    CAS  PubMed  Google Scholar 

  23. Garen, A., and Levinthal, C., Biochim. Biophys. Acta, 38, 470 (1960).

    Article  CAS  PubMed  Google Scholar 

  24. Schachman, H. K., Ultracentrifugation in Biochemistry, 247 (Academic Press, New York, 1959).

    Google Scholar 

  25. Studier, F. W., and Maizel, jun., J. V., Virology, 39, 575 (1969).

    Article  CAS  PubMed  Google Scholar 

  26. Siegel, R. B., and Summers, W. C., J. Mol. Biol., 49, 115 (1970).

    Article  CAS  PubMed  Google Scholar 

  27. Gillespie, D., and Spiegelman, S., J. Mol. Biol., 12, 829 (1965).

    Article  CAS  PubMed  Google Scholar 

  28. Walter, G., Seifert, W., and Zillig, W., Biochem. Biophys. Res. Commun., 30, 240 (1968).

    Article  CAS  PubMed  Google Scholar 

  29. Roberts, J., Nature, 223, 480 (1969).

    Article  ADS  CAS  PubMed  Google Scholar 

  30. Salser, W., Bolle, A., and Epstein, R., J. Mol. Biol., 49, 271 (1970).

    Article  CAS  PubMed  Google Scholar 

  31. Losick, R., and Sonenshein, A. L., Nature, 224, 35 (1969).

    Article  ADS  CAS  PubMed  Google Scholar 

  32. Pulitzer, J. F., and Geiduschek, E. P., J. Mol. Biol., 49, 489 (1970).

    Article  CAS  PubMed  Google Scholar 

  33. Skalka, A., Butler, B., and Echols, H., Proc. US Nat. Acad. Sci., 58, 576 (1967).

    Article  ADS  CAS  Google Scholar 

  34. Takeda, Y., Oyama, Y., Nakajima, K., and Yura, T., Biochem. Biophys. Res. Commun., 36, 533 (1969).

    Article  CAS  PubMed  Google Scholar 

  35. Haselkorn, R., Vogel, M., and Brown, R., Nature, 221, 836 (1969).

    Article  ADS  CAS  PubMed  Google Scholar 

  36. Butler, B., and Echols, H., Virology, 40, 212 (1970).

    Article  CAS  PubMed  Google Scholar 

  37. Rabovsky, D., and Konrad, M., Virology, 40, 10 (1970).

    Article  CAS  PubMed  Google Scholar 

  38. Konrad, M., Proc. US Nat. Acad. Sci., 59, 171 (1968).

    Article  ADS  CAS  Google Scholar 

  39. Schwartz, M., Virology, 40, 23 (1970).

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

CHAMBERLIN, M., MCGRATH, J. & WASKELL, L. New RNA Polymerase from Escherichia coli infected with Bacteriophage T7. Nature 228, 227–231 (1970). https://doi.org/10.1038/228227a0

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1038/228227a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing