Abstract
WE have made use of micro-cannulation—a new and potentially useful technique for plant physiology—by inserting glass micropipettes of 2–4 µm tip diameter into mature living sieve tubes of Heracleum sphondylium. We used a micromanipulator1 under a microscope with a Zeiss × 40 water immersion objective. The technique in its present state of development may disturb the sieve element and so alter normal translocation, but it has potential advantages over the use of aphid stylets2,3, for the glass is impermeable to water, the tips can be located precisely in situ and injection of solutes as well as withdrawal of sap are possible.
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FENSOM, D., DAVIDSON, H. Micro-injection of 14C-Sucrose into Single Living Sieve Tubes of Heracleum. Nature 227, 857–858 (1970). https://doi.org/10.1038/227857a0
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DOI: https://doi.org/10.1038/227857a0
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