Abstract
WHILE investigating the bactericidal action of the basic proteins released from the lysosomes of rabbit polymorphonuclear leucocytes, we found that the rate of destruction of staphylococci was influenced by the medium in which the test was carried out. When a chemically defined medium1 (containing phosphate buffer at pH 7.4, glucose, seventeen amino-acids, thiamine, nicotinamide and salts of Mg2+, Mn2+, Fe2+ or Fe3+) was used, the bactericidal action of the leucocyte extracts was markedly inhibited and in certain cases abolished. The inhibition was due to the presence of Fe2+ or Fe3+.
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GLADSTONE, G., WALTON, E. Effect of Iron on the Bactericidal Proteins from Rabbit Polymorphonuclear Leucocytes. Nature 227, 849–851 (1970). https://doi.org/10.1038/227849a0
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DOI: https://doi.org/10.1038/227849a0
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