Molar Absorbance Coefficient of Rhodopsin

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UNTIL recently, it was accepted that the molar absorbance coefficient of rhodopsin, εrh, was no less than 41,000 (refs. 1–4). In 1968, however, Heller5 obtained a value of 23,100 by measuring the amount of protein in purified samples of rhodopsin and assuming a protein molecular weight of 26,400.

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    Shichi, H., Lewis, M. S., Irreverre, F., and Stone, A. L., J. Biol. Chem., 244, 529 (1969).

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    Heller, J., Biochemistry, 7, 2906 (1968).

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    Shields, J. E., Dinovo, E. C., Henrikson, R. A., Kimbel, R. L., and Millar, P. G., Biochim. Biophys. Acta, 147, 238 (1967).

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BRIDGES, C. Molar Absorbance Coefficient of Rhodopsin. Nature 227, 1258–1259 (1970) doi:10.1038/2271258a0

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