Conformation of Actinomycin-D

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NUCLEAR magnetic resonance (NMR) spectroscopy has recently been found useful for investigating the conformations of polypeptides like gramicidin S1,2, valinomicin3 and so on. We now report preliminary conclusions on the conformation of the two peptide lactone rings of actinomycin D4 on the basis of NMR results and theoretical conformational analysis. Fig. 1 depicts the NMR spectra of actinomycin D obtained in different experimental conditions. The assignment has been made on the basis of chemical shift values, and the characteristics of the signals in double and triple resonance experiments. By examining the results obtained, some interesting features can be derived.

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  1. 1

    Sterm, A., Gibbons, W. A., and Craig, L. C., Proc. US Nat. Acad. Sci., 61, 735 (1968).

  2. 2

    Conti, F., Nature, 221, 777 (1969).

  3. 3

    Finer, E. G., Chapman, D., and Hauser, H., Proc. Intern. Symp. Magnetic Resonance in Biological Research, Cagliari, 1969.

  4. 4

    Brockman, H., and Boldt, P., Chem. Ber., 101, 1940 (1968).

  5. 5

    Victor, F. A., Hruska, F. E., Hikichi, K., Danyluk, S. S., and Bell, C. L., Nature, 223, 302 (1969).

  6. 6

    Bovey, F. A., Proc. IUPAC Symp. Macromolecular Chemistry, Brussels (1967).

  7. 7

    Goodman, M., and Fried, M., J. Amer. Chem. Soc., 89, 1264 (1967).

  8. 8

    Conti, F., and De Santis, P., Biopolymers (in the press).

  9. 9

    Conti, F., Piattelli, M., and Viglino, P., Biopolymers, 7, 411 (1969).

  10. 10

    Muller, W., and Emmie, I., Z. Naturforsch., 20, 835 (1965).

  11. 11

    Bystrof, V. F., Portnove, S. L., Tsetlen, V. I., Ivanov, V. I., and Ovchinnikov, Yu. A., Tetrahedron, 25, 493 (1969).

  12. 12

    Palmer, H. J., Palmer, R. A., and Dickerson, R. E., Nature, 202, 1052 (1964).

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CONTI, F., DE SANTIS, P. Conformation of Actinomycin-D. Nature 227, 1239–1241 (1970) doi:10.1038/2271239a0

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