Solvent Effect on the Conformation of Actinomycin D

Article metrics

Abstract

THERE have been several different approaches to the stereochemistry of the interaction of actinomycin and DNA, including studies of the interaction of DNA-like polynucleotides with actinomycin1,2, and of DNA with model compounds related to actinomycin3–5, using various experimental techniques. Reports on the optical rotatory-dispersion (ORD) and circular dichroism (CD) data for the actinomycin–DNA complex and for actinomycin alone6–9, as well as unpublished results from this laboratory, indicate that the rotational strength of the optically active transitions—which can be ascribed to the dimethylaminophenoxazone chromophore of actinomycin—in the visible region of the spectrum increases in the presence of DNA. The molecular mechanism of the interaction of actinomycin with DNA is still obscure, however, for the increase in optical activity could result from a change in the conformation of actinomycin in the presence of DNA and/or from the perturbing influence of the asymmetric environment (DNA) on the chromophore transitions of actinomycin.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Reich, E., and Goldberg, I. H., in Prog. Nucleic Acid Res. Mol. Biol. (edit. by Davidson, J. N., and Cohen, W. E.), 1, 183 (Academic Press, New York, 1968).

  2. 2

    Cerami, A., Reich, E., Ward, D. C., and Goldberg, I. H., Proc. US Nat. Acad. Sci. 57, 1036 (1967).

  3. 3

    Ascoli, F., Savino, M., and Liquori, A. M., Nature, 217, 162 (1968).

  4. 4

    Ascoli, F., and Savino, M., Nature, 217, 162 (1968).

  5. 5

    Müller, W., and Crothers, D. M., J. Mol. Biol., 35, 251 (1968).

  6. 6

    Ziffer, H., Yamaoka, K., and Mauger, A., Biochemistry, 7, 996 (1968).

  7. 7

    Yamaoka, K., and Ziffer, H., Biochemistry, 7, 1001 (1968).

  8. 8

    Crothers, D. M., Sabol, S. L., Ratner, D. I., and Müller, W., Biochemistry, 7, 1817 (1968).

  9. 9

    Homer, R. B., Arch. Biochem. Biophys., 129, 405 (1969).

  10. 10

    Moscowitz, H., in Optical Rotatory Dispersion and Circular Dichroism in Organic Chemistry (edit. by Snatzke, G.), 41 (Heyder, London, 1967).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

ASCOLI, F., DE SANTIS, P. & SAVINO, M. Solvent Effect on the Conformation of Actinomycin D. Nature 227, 1237–1239 (1970) doi:10.1038/2271237a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.