Asparaginyl-tRNA and Resistance of Murine Leukaemias to L-Asparaginase

Article metrics


INTEREST in the enzyme L-asparaginase has been stimulated by the observation that it inhibits the growth of experimental and human tumours1–3. Tumours sensitive to L-asparaginase require exogenous L-asparagine whereas tumours not sensitive synthesize L-asparagine from L-aspartic acid and L-glutamine. Moreover, if L-asparaginase-sensitive tumours are made resistant using suboptimal doses of the enzyme, the resistant variant possesses L-asparagine synthetase and no longer requires exogenous L-asparagine for growth3,4. The critical questions now concern the regulation of L-asparagine synthetase activity.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    Kidd, J. G., J. Exp. Med., 98, 565 (1953).

  2. 2

    Old, L. J., Boyse, B. A., Campbell, H. A., Brodey, R. S., Fidler, J., and Teller, J. D., Cancer, 20, 1066 (1967).

  3. 3

    Adamson, R. H., and Fabro, S., Cancer Chemother. Rep., 52, 617 (1968).

  4. 4

    Horowitz, B., Madras, B. K., Meister, A., Old, L. J., Boyse, E. A., and Stockert, E., Science, 160, 533 (1968).

  5. 5

    Duda, E., Staub, M., Venetianer, P., and Denes, G., Biochem. Biophys. Res. Commun., 32, 992 (1968).

  6. 6

    Schlesinger, S., and Magasanik, B., J. Mol. Biol., 9, 670 (1964).

  7. 7

    Eidlic, L., and Neidhardt, F. C., Proc. US Nat. Acad. Sci., 47, 56 (1965).

  8. 8

    Silbert, D. F., Fink, G. R., and Ames, B. N., J. Mol. Biol., 22, 335 (1966).

  9. 9

    Stulberg, M. P., Isham, K. R., and Stevens, A., Biochim. Biophys. Acta, 186, 297 (1969).

  10. 10

    Kirby, K. S., Biochem. J., 64, 405 (1956).

  11. 11

    Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. Biol. Chem., 193, 265 (1951).

  12. 12

    Weiss, J. F., and Kelmers, A. D., Biochemistry, 6, 2507 (1967).

  13. 13

    Gallo, R. C., J. Cell. Physiol., 74, suppl. 1, 149 (1969).

  14. 14

    Yang, W. K., and Novelli, G. D., Proc. US Nat. Acad. Sci., 59, 208 (1968).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

GALLO, R., LONGMORE, J. & ADAMSON, R. Asparaginyl-tRNA and Resistance of Murine Leukaemias to L-Asparaginase. Nature 227, 1134–1136 (1970) doi:10.1038/2271134a0

Download citation

Further reading


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.