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Total Synthesis of the Gene for an Alanine Transfer Ribonucleic Acid from Yeast  

Abstract

By exploiting the natural ability of polynucleotides to align by base pairing and using polynucleotide kinase and ligase, chemically synthesized segments have been combined into a double stranded DNA corresponding to the gene for the earliest characterized tRNA.

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References

  1. Khorana, H. G., Pure Appl. Chem., 17, 349 (1968).

    CAS  Article  Google Scholar 

  2. Khorana, H. G., Proc. Seventh Intern. Cong. Biochem., Tokyo, 1967, 17 (1968).

    Google Scholar 

  3. Holley, R. W., Apgar, J., Everett, G. A., Madison, J. T., Marquisee, M., Merrill, S. H., Penswick, J. R., and Zamir, A., Science, 147, 1462 (1965).

    ADS  CAS  Article  Google Scholar 

  4. Gupta, N. K., Ohtsuka, E., Weber, H., Chang, S. H., and Khorana, H. G., Proc. US Nat. Acad. Sci., 60, 285 (1968).

    ADS  CAS  Article  Google Scholar 

  5. Gupta, N. K., Ohtsuka, E., Sgaramella, V., Buchi, H., Kumar, A., Weber, H., and Khorana, H. G., Proc. US Nat. Acad. Sci., 60, 1338 (1968).

    ADS  CAS  Article  Google Scholar 

  6. Gupta, N. K., and Khorana, H. G., Proc. US Nat. Acad. Sci., 61, 215 (1968).

    ADS  CAS  Article  Google Scholar 

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AGARWAL, K., BÜCHI, H., CARUTHERS, M. et al. Total Synthesis of the Gene for an Alanine Transfer Ribonucleic Acid from Yeast  . Nature 227, 27–34 (1970). https://doi.org/10.1038/227027a0

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