Abstract
By exploiting the natural ability of polynucleotides to align by base pairing and using polynucleotide kinase and ligase, chemically synthesized segments have been combined into a double stranded DNA corresponding to the gene for the earliest characterized tRNA.
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References
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AGARWAL, K., BÜCHI, H., CARUTHERS, M. et al. Total Synthesis of the Gene for an Alanine Transfer Ribonucleic Acid from Yeast . Nature 227, 27–34 (1970). https://doi.org/10.1038/227027a0
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DOI: https://doi.org/10.1038/227027a0
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