Active Water-insoluble Derivative of Renin

Article metrics

Abstract

ALTHOUGH the action of renin on the blood pressure has been explored by many investigators, some of its basic enzymatic properties are yet to be established1. While studying plasma kinins, we found it to be of advantage to couple enzymes that release or inactivate kinins covalently to water-insoluble complexes2–4. The active insoluble derivatives of enzymes are convenient to use, because, among many other attractive properties, they are stable, re-usable and readily removed from incubation mixture by sedimentation. We have extended these investigations to renin. Our previous results with killikrein, carboxypeptidases, kallikrein and trypsin inhibitors and renin were briefly reported3,4. This report deals with the binding of renin to ‘Sepharose 4B’.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Renal Hypertension (edit. by Page, I. H., and McCubbin, J. W.) (Year Book Medical Publishers, Inc., Chicago, 1968).

  2. 2

    Erdös, E. G., Seki, T., Yang, H. Y. T., and Tague, L. L., Pharmacol. Res. Commun., 1, 152 (1969).

  3. 3

    Seki, T., Yang, H. Y. T., Levin, Y., Jenssen, T. A., and Erdös, E. G., in International Symposium on Cardiovascular and Neuro-actions of Bradykinin and Related Kinins (Fiesole-Florence, July 21–25, 1969).

  4. 4

    Seki, T., Yang, H. Y. T., Levin, Y., Jenssen, T. A., and Erdös, E. G., Advances in Experimental Medicine and Biology (in the press).

  5. 5

    Sen, S., Smeby, R. R., and Bumpus, F. M., Biochemistry, 6, 1572 (1967).

  6. 6

    Green, A. A., and Bumpus, F. M., J. Biol. Chem., 210, 281 (1954).

  7. 7

    Chiang, T. S., Erdös, E. G., Miwa, I., Tague, L. L., and Coalson, J. J., Circ. Res., 23, 507 (1968).

  8. 8

    Axen, R., Porath, J., and Ernback, S., Nature, 214, 1302 (1967).

  9. 9

    Cuatrecasas, P., Wilchek, M., and Anfinsen, C. B., Proc. US Nat. Acad. Sci., 61, 636 (1968).

  10. 10

    Regoli, D., and Vane, J. R., Brit. J. Pharmacol. Chemother., 23, 351 (1964).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

SEKI, T., JENSSEN, T., LEVIN, Y. et al. Active Water-insoluble Derivative of Renin. Nature 225, 864–865 (1970) doi:10.1038/225864a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.